In vitro propagation of Caladium bicolor with different doses of growth regulators
DOI:
https://doi.org/10.33064/iycuaa2021833749Keywords:
Plant tissue culture, organogenesis, bulbous plants, auxins, cytokinins, ornamental plantsAbstract
Caladium bicolor is an ornamental crop used to beautify the urban landscape, squares and gardens and is exported to other countries. The objective was to develop an in vitro propagation system for Caladium bicolor by organogenesis. The growth regulators 6-benzylaminopurine (BAP) and naphthaleneacetic acid (ANA) were added to the basal MS medium with four doses each: 1.0:0.10, 1.5:0.15, 2.0:0.20 and 2.5:0.25 mg L-1 of BAP:ANA for the shoot production stage and 0.10:1.0, 0.15 1.5, 0.20:2.0 and 0.25:2.5 mg L-1 of BAP:ANA for the rooting stage. The dose of 1.5:0.15 mg L-1 of BAP:ANA showed a better response in the production of shoots and leaves, and in the rooting stage the dose with 0.15:1.5 mg L-1 of BAP:ANA presented plants with a higher number and root length. These doses presented plants with more balanced vegetative components, allowing to obtain strengthened plants for the acclimatization stage.
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Copyright (c) 2021 Ixchel Abby Ortiz-Sánchez, Jorge Armando Chávez-Simental, Cecilia Pulido-Díaz, Erika Cecilia Gamero-Posada, Sonia Valdez-Ortega, Guillermo González-Cervantes
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