Fertilización in vitro (FIV) de ovocitos obtenidos en ovejas mediante la técnica de recolección laparoscópica de óvulos

Julio Ramón Ugalde; Deneb Cervera Paúl; Álvaro Domínguez Rebolledo; Juan Baeza-Rodríguez; Luis Pinzón-López; Roberto Zamora-Bustillos

doi:10.33064/iycuaa2020813137

Authors

Julio Ramón Ugalde Instituto Tecnológico de Conkal http://orcid.org/0000-0002-9312-8438
Deneb Cervera Paúl Instituto Tecnológico de Conkal http://orcid.org/0000-0002-4508-9462
Álvaro Domínguez Rebolledo Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias campo experimental Mocochá http://orcid.org/0000-0002-1444-3844
Juan Baeza-Rodríguez Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias campo experimental Mocochá http://orcid.org/0000-0001-9339-9695
Luis Pinzón-López Tecnológico Nacional de México campus Conkal http://orcid.org/0000-0002-8405-3948
Roberto Zamora-Bustillos Tecnológico Nacional de México campus Conkal http://orcid.org/0000-0002-4502-1492

DOI:

https://doi.org/10.33064/iycuaa2020813137

Keywords:

sheep, oocytes, embryos, biotech, maturation, laparoscopy

Abstract

In order to fine-tune the laparoscopic ovum pick up (LOPU) technique and develop an in vitro fertilization (IVF) protocol, two groups of sheep were synchronized (vaginal sponges 14 days) and superovulated (FSHp), beginning 60 hours before each LOPU (3 sessions). Laparoscopic follicular aspiration was performed 12 hours after the last dose of FSHp. The overall recovery rate of oocytes in three LOPU sessions in G2 and G1 was 41.0 ± 2.5 and 24.0 ± 2.4% SEM, respectively, presenting differences (P<0.05) in favor of G2. In vitro oocyte culture at 48 hours showed a difference (P<0.05) in favor of G1 (9.7 %) with respect to G2 (0.0).

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