Study of effects in pH, stomatal density, índole-3-acetic acid and abscisic acid on cacti cultivated in vitro and ex vitro
DOI:
https://doi.org/10.33064/iycuaa2023884089Keywords:
cacti, Crassulacean Acid Metabolism, stomata, photosynthesis, phytohormonesAbstract
Crassulaceae acid metabolism (MAC or CAM) generally takes place in plants that live in arid areas with little water availability. These plants are characterized by being succulent, having a waxy cuticle and carrying out photosynthesis in the stems. In this work several aspects of its metabolism in cacti in vitro and ex vitro are addressed. The variation of the pH was analyzed every hour during a day and it was observed that Opuntia ficus-indica in vitro under continuous light present a C3 behavior.. The study of the stomata made it possible to relate the parallelocytic type with the Cactoideae subfamily, and the opuntoid type with the Pereskioideae and Opuntioideae subfamilies in cacti grown in vitro. Further, a stimulation in the stomatal opening was observed in O. ficus-indica and P. sacharosa with indoleacetic acid, only at high concentrations (100 and 1000 µM).
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