Reactivity of polyclonal antibodies toward glycomacropeptide of bovine cheese whey in front of the glycomacropeptide of cheese whey from sheep

Authors

  • Norma Angélica Chávez Vela Universidad Autónoma de Aguascalientes https://orcid.org/0000-0003-0932-5402
  • Eva Salinas Miralles Universidad Autónoma de Aguascalientes
  • Juan Jáuregui Rincón Universidad Autónoma de Aguascalientes https://orcid.org/0000-0002-2584-1661
  • Fernando Bon Rosas Universidad Autónoma de Aguascalientes
  • Dulce María Diana Pérez Tellez Universidad Autónoma de Aguascalientes

DOI:

https://doi.org/10.33064/iycuaa2012544071

Keywords:

glycomacropeptide, polyclonal antibodies, immuno blot, cheese whey, sheep milk

Abstract

The production and consumption of milk from sheep in Mexico and the world, is increasing every time because this has important advantages over cow’s milk. A common practice of milk producers is its adulteration with cheese whey. However, this affects milk processor industries because it decreases the yield of the products obtained. There are several methods for determining the presence of cheese whey based on the detection of a glycomacropeptide (GMP) that is present only in SQ and not in milk. However, none of these methods is focused on the detection of sheep’s GMP. In this study, polyclonal antibodies toward anti-glycomacropeptide bovine were used to determine the reactivity of these in front to the GMP of cheese whey from sheep as an indicator of the presence of cheese whey. 

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Author Biographies

Norma Angélica Chávez Vela, Universidad Autónoma de Aguascalientes

Departamento de Ingeniería Bioquímica, Centro de Ciencias Básicas

Eva Salinas Miralles, Universidad Autónoma de Aguascalientes

Departamento de Microbiología, Centro de Ciencias Básicas

Juan Jáuregui Rincón, Universidad Autónoma de Aguascalientes

Departamento de Ingeniería Bioquímica, Centro de Ciencias Básica

Fernando Bon Rosas, Universidad Autónoma de Aguascalientes

Departamento de Ingeniería Bioquímica, Centro de Ciencias Básica

Dulce María Diana Pérez Tellez, Universidad Autónoma de Aguascalientes

Departamento de Ingeniería Bioquímica, Centro de Ciencias Básicas

References

• ALAVA, M.; CALVO M.; INDA, L.; RAZQUÍN, P.; LAMPREAVE, F., Rapid, sensitive, enzyme-immunodotting assay for detecting cow milk adulteration in sheep milk: a modern laboratory project. J Chem Educ., 75(12): 16-18, 1998.

• ALCÁZAR, M.C.; ROSAS, J.; JARAMILLO, A.C.; PEÑA, S., Detección de glucomacropéptido (GMP) como indicador de adulteración con suero de quesería en leche deshidratada. Vet México, 37(3) 217-222, 2000.

• BAIN, I., Elaboración de quesos artesanales con leche de oveja. IDIA XXI, 7: 208-2011, 2004.

• BENÍTEZ, E.; PONCE, P.; NOA, M., Detección de suero de quesería en leche en polvo por HPLC de filtración. Gel. Rev. Salud Animal, 23(1): 27-31, 2001.

• BREMER, M.; KEMMERS VONCKEN, A.; BOERS, E.; FRANKHUIZEN, R.; HAASNOOT, W., Enzyme-Linked immunosorbent assay for the detection of bovine rennet whey powder in milk powder and buttermilk powder. Int Dairy J. 18: 294-302, 2008.

• BRODY, E.P., Biological activities of bovine glycomacropeptide. Br J Nutr., 84(1): S39-S46, 2000.

• CHÁVEZ, N.; SALINAS, E.; JÁUREGUI, J.; PALOMARES, L.A.; MACÍAS, K.; Detection of bovine milk adulterated with cheese whey by Western blot immunoassay. Food Agric Immunol, 19: 265-272, 2008.

• CHÁVEZ, N.; JÁUREGUI, J.; PALOMARES, L.; MACÍAS, K.; JIMÉNEZ, M.; SALINAS, E., A highly sensitive sandwich ELISA for the determination of glycomacropeptide to detect liquid whey in raw milk. Dairy Sci. & Technol., 92, 121-132, 2012.

• COSTA, N.; RAVASCO, F.; MIRANDA, R.; DUTHOIT, M.; ROSEIRO, L.B., Evaluation of a commercial ELISA method for the quantitative detection of goat and cow milk in ewe milk and cheese. Small Ruminant Res, 79, (1): 73-79, 2008.

• GALINDO, L.; VALBUENA, E.; ROJAS, E., Estandarización de la detección del glicomacropéptido por PAGESDS como índice de adulteración de leche. Rev Cient FCV-LUZ, 16(3): 308-314, 2006.

• HAZA, I.; MORALES, P.; MARTIN, R.; GARCÍA, T.; ANGUITA, G.; GONZÁLEZ I.; SANZ, B.; HERNÁNDEZ, P., Development of monoclonal antibodies against caprine αs2-casein and their potential for detecting the substitution of ovine milk by caprine milk by an indirect elisa.

J Agric Food Chem, 44: 1756-1761, 1996.

• HOLDEN, L.; FÆSTE, C.; EGAAS, E., Quantitative Sandwich ELISA for determination of Lupine (Lupinus spp.) in foods. j Agric Food Chem, 53: 5866-5871, 2005.

• HONGXIN, S.; HAIYAN, X.; YAN, H., Detection of cow’s milk in shaanxi goat’s milk with an elisa assay. Food Control, 22: 883-88, 2011.

• LÓPEZ CALLEJA, I.; GONZÁLEZ A.; FAJARDO, V.; RODRÍGUEZ, M; HERNÁNDEZ, P.E.; GARCÍA, T., Application of polymerasechain reaction to detect adulteration of sheep’s milk with goat’s milk. J. Dairy Sciences, 88:3115-3120, 2005.

• LÓPEZ FANDIÑO, M; ACEDO, M.I.; RAMOS, M., Comparative study by HPLC of caseinnomacropeptides from cows´ewes´ and goats´ milk. J Dairy Res, 60: 117-121,1993.

• MOATSOU, G; ANIFANTAKIS, E., Recent developments in antibody-based analytical methods for the differentiation of milk from different species. Int J Dairy Technol., 56(3): 133-138, 2003.

• MOLINA, E., ÁLVAREZ, M.; RAMOS, M., Milk species identification in cheese varieties using electrophoretic, chromatographic and PCR techniques. Int. Dairy J., 9: 99-105, 2005.

• OANCEA, S., Identification of glycomacropeptide as indicator of milk and dairy drinks adulteration with whey by mmunochromatographic assay. Rom Biotechnol Lett, 14: 4146-4151, 2009.

• PARK, Y.W.; JUÁREZ, M.; RAMOS, M.; HAENLEIN, G.F.W., Physico-chemical characteristics of goat and sheep milk. Small Ruminant Res, 68: 88-113, 2007.

• RECIO, I.; GARCÍA RISCO, M.R.; RAMOS, M.; LÓPEZ FANDIÑO, R., Characterization of peptides produced by the action of psychrotrophic proteinase on k-casein. J Dairy Res, 67: 625-630, 2000.

• SANZ, M.R.; FERNÁNDEZ, J.R.; DE LA TORRE, G.; RAMOS, E.; CARFONA, F.D.; BOZA, J., Calidad de la leche de los pequeños rumiantes. Anales de la Real Academia de Ciencias Veterinarias de Andalucía Oriental, 16(1):155-166, 2003.

• SILVA HERNÁNDEZ, E.R.; NAKANO, T.; VERDALETGUZMÁN, I., OZIMEK, L., Comparison of glycomacropeptide isolated from raw and pasteurized goat milk.,Milchwissenschaft-milk Sci In., 59: 27-31, 2004. Dictiotipografía

• FAO, Base de datos TRADESTAT. Disponible en: www.fao.org. Consultado en, Diciembre de 2011.

• TREJO, G.E., Leche ovina, un negocio que da lana, El economista. Disponible en: http://eleconomista.com.mx/notas-impreso/columnas/agro-negocios/2009/04/16/leche-ovina-negocio-mexico. Consultado en diciembre de 2011.

Published

2012-04-30

How to Cite

Chávez Vela, N. A., Salinas Miralles, E., Jáuregui Rincón, J., Bon Rosas, . F., & Pérez Tellez, D. M. D. (2012). Reactivity of polyclonal antibodies toward glycomacropeptide of bovine cheese whey in front of the glycomacropeptide of cheese whey from sheep. Investigación Y Ciencia De La Universidad Autónoma De Aguascalientes, (54), 12–16. https://doi.org/10.33064/iycuaa2012544071

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Artículos de Investigación

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