In vitro propagation of wild laurel (Litsea glaucescens Kunth) and analysis of genetical diversity of central Mexican populations
DOI:
https://doi.org/10.33064/iycuaa2013574012Keywords:
Lauraceae, RAPD, phylogenetic tree, OPA, PCR, analysis of similarity, Euclidean distancesAbstract
The wild Laurel (Litsea glaucescens Kunth) is a forestry species considered an endangered species due to the reduction of their population by extensive and illegal collections of their branches for different uses. In this study several populations of wild Laurel in Central Mexico (Aguascalientes and San Luis Potosí States), were localized and studied. The most effective protocol for propagation was obtained from explants generated of the germination of in vitro mature seeds, with 0.5 mg L-1 BA. For plant rooting the best treatment was that of 1 mg L-1 AIB. The dendrogram showed a separation between a group that included only specimens from San
Luis Potosí, and a second group with specimens from San Luis Potosí but also specimens from Aguascalientes. The specimens generated in vitro did not split from the rest and were kept inside the group from the location which they were initially from.
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• ALFARO ROJAS, G.; LEGARIA SORIA J.P.; RODRÍGUEZ PÉREZ, J.E., Genetic diversity in populations of pulqueros agaves (Agave spp.) in Noretheastern México State. Revista Fitotecnia Méxicana, 30: 1-12, 2007.
• BADENES, M.; GARCÉS, A.; ROMERO, C.; ROMERO, M.; CLAVE, J.; ROVIRA M.; LLACÉR, G., Genetic diversity of introduced and local Spanish persimmon cultivars revealed by RAPD markers. Genetic Resources and Crop Evolution, 50: 579-585, 2003.
• BUNN, E., Development of in vitro methods for ex situ conservation of Eucalyptus impensa, an endangered mallee from southwest Western Australia. Plant Cell, Tissue and Organ Culture, 83: 97-102, 2005.
• DÁVILA, F.C.A.; FLORES, T.F.J.; MORALES, D.J.F.; CLARK, T.R.; PÉREZ, M.B.E., Estatus poblacional y niveles de aprovechamiento del laurel silvestre (litsea glaucescens kunth) en Aguascalientes. Revista Mexicana de
Ciencias Forestales, 2(4): 47-60, 2011.
• GOMES, F.; CANHOTO J.M., Micropropagation of Eucalyptus nitens Maiden (shining gum). In Vitro Cell. Dev.
Biol.—Plant, 39: 316-321, 2003.
• GUPTA, P.K.; MEHTA, U.J.; MASCARENHAS, A.F., A Tissue Culture Method for Rapid Clonal Propagation of Mature Trees of Eucalyptus toreiliana and Eucalyptus camaldulensis. Plant Cell Reports, 2: 296-299, 1983.
• LARA CABRERA, S.I.; ALEJANDRE MELENA, N.; MEDINA SÁNCHEZ, E.I.; LINDIG CISNEROS, R., Genetic diversity in populations of Lupinus elegans Kunth, implications for LITERATURA CITADA ecological restoration. Revista Fitotecnia Mexicana, 32: 79-86, 2009.
• LUNA RODRÍGUEZ, M.; LÓPEZ UPTO, J.; IGLESIAS ANDREU, L.G., Morphometric and molecular (RAPD) variability in a plantation of Pinus patula in Veracruz, México. Agrociencia, 39: 231-235, 2005.
• MAO, A.A.; WETTEN, A.; FAY, M.F.; CALIGARI, P.D., In vitro propagation of Litsea cubeba (Lours.) Pers., a multipurpose tree. Plant Cell Reports, 19: 263-267, 2000.
• MURASHIGE, T.; SKOOG, F., A revised medium for rapid growth and bioassays with tobacco cultures. Physiol. Plant, 15: 473, 1962.
• PROESPA, 2008. Especies protegidas en el estado de Aguascalientes caso: Sierra de Laurel en el municipio de Calvillo. http://www.proespa.gob.mx/pfnm/index.html.
• RZEDOWSKI, G.; RZEDOWSKI, J., Flora Fanerogámica del Valle de México. Michoacán: Instituto de Ecología. A. C. y CONABIO, 2001).
• VASIL, I.K., Cell Culture and Somatic Cell Genetics of Plants. USA: Advisory Board, Academic Press, Inc., 1986.
• WILLIAMS, J.G.; KUBELIK, A.R.; LIVAK, K.J.; RAFALSKI J.A.; TINGEY, S.V., DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Research, 18: 6531-6535, 1990.
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Copyright (c) 2013 Claudia Montserrat Valle Rodríguez, Carlos Antonio Dávila Figueroa, María de Lourdes de la Rosa Carrillo, Eugenio Pérez Molphe-Balch, José Francisco Morales Domínguez
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