Effect of chitosan on the induction of disease resistance against Colletotrichum sp. in mango (Mangifera indica L.) cv. Tommy Atkins

Authors

  • Guillermo Berumen Varela Instituto Tecnológico de Tepic
  • Leonardo Daniel Coronado Partida Instituto Tecnológico de Tepic
  • Verónica Alhelí Ochoa Jiménez Instituto Tecnológico de Tepic
  • Martina Alejandra Chacón López Instituto Tecnológico de Tepic
  • Porfirio Gutiérrez Martínez Instituto Tecnológico de Tepic

DOI:

https://doi.org/10.33064/iycuaa2015663565

Keywords:

mango, chitosan, induction of disease resistance, peroxidase, Colletotrichum sp

Abstract

Mango is susceptible to a postharvest disease called anthracnose, caused by pathogen Colletotrichum sp. Chemical fungicides have been used against postharvest fungal diseases, but they cause damage to the environment. Chitosan shows antimicrobial properties, and induces resistance in plants highlighting the activity of some enzymes like peroxidase. The objective of this research was to evaluate the chitosan effect in the induction of resistance mechanism against Colletotrichum sp., in mango fruits. Chitosan inhibited spore germination, germ tube elongation, and mycelial growth of Colletotrichum sp. in vitro. For postharvest fruits the
chitosan at 1.0% and 1.5% Colletotrichum sp. was completely inhibited in fruits inoculated with the fungi. 

Downloads

Download data is not yet available.

Metrics

Metrics Loading ...

Author Biographies

Guillermo Berumen Varela, Instituto Tecnológico de Tepic

Laboratorio Integral de Investigación en Alimentos-Laboratorio de
Biotecnología

Leonardo Daniel Coronado Partida, Instituto Tecnológico de Tepic

Laboratorio Integral de Investigación en Alimentos-Laboratorio de
Biotecnología

Verónica Alhelí Ochoa Jiménez, Instituto Tecnológico de Tepic

Laboratorio Integral de Investigación en Alimentos-Laboratorio de
Biotecnología

Martina Alejandra Chacón López, Instituto Tecnológico de Tepic

Laboratorio Integral de Investigación en Alimentos-Laboratorio de
Biotecnología

Porfirio Gutiérrez Martínez, Instituto Tecnológico de Tepic

Laboratorio Integral de Investigación en Alimentos-Laboratorio de
Biotecnología

References

• ARAUZ, L. F. Mango anthracnose: Economic impact and current options for integrated management. Plant Disease, 84(6): 600‐611, 2000.

• BARNET, H., y HUNTER, B. Illustrated genera of imperfect fungi. New York: MacMillan Publishing Co., 200-201, 1998.

• BAUTISTA BAÑOS S. et al. Effects of chitosan and plant extracts on growth of Colletotrichum gloeosporioides, anthracnose levels and quality of papaya fruit. Crop protection, 22(9): 1087-1092, 2003.

• BENHAMOU, N. et al. Induction of systemic resistance to Fusarium crown and root rot in tomato plants by seed treatment with chitosan. Phytopathology, 84, 1432–1444, 1994.

• BEN-SHALOM, N. et al. Controlling gray mould caused by Botrytis cinerea in cucumber plants by means of chitosan. Crop Protection, 22(2): 285-290, 2003.

• BHASKARA, R. M. et al. Effect of pre-harvest chitosan sprays on post-harvest infection by Botrytis cinerea and quality of strawberry fruit. Postharvest Biology and Technology, 20(1):, 39-51, 2000.

• BRADFORD, M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry, 72(1-2): 248-254, 1976.

• CHEN, C. et al. Defense enzymes induced in cucumber roots by treatment with plant growth-promoting rhizobacteria (PGPR) and Pythium aphanidermatum. Physiology and Molecular Plant Pathology, 56(1): 13-23, 2000.

• EL GHAOUTH, A. et al. Antifungal activity of chitosan on two postharvest pathogens of strawberry fruits. Phytopathology, 82, 398-402, 1992.

• EL GHAOUTH, J. et al. Chitosan coating effect on storability and quality of fresh strawberries. Journal of Food Science, 56(6): 1618-1620, 1991.

LITERATURA CITADA

• EL HADRAMI, A. et al. Chitosan in Plant Protection. Marine Drugs, 8(4): 968-987, 2010.

• KOLATTUKUDY, P. E. et al. Plant peroxidase gene expression and function. Biochemical Society Transactions, 20(2): 333-337, 1992.

• LIU, J. et al. Effects of chitosan on control of postharvest diseases and physiological responses of tomato fruit. Postharvest Biology

and Technology, 44(3): 300-306, 2007.

• LIN, J. et al. Expression of PPO and POD genes and contents of polyphenolic compounds in harvested mango fruits in relation to Benzothiadiazole-induced defense against anthracnose. Scientia Horticulturae, 130(1): 85-89, 2011

• LÓPEZ GÓMEZ, R. y GÓMEZ LIM, M. A. A method for extracting intact RNA from fruits rich in polysaccharides using ripe mango mesocarp. Horticultural Science, 27(5): 440-442, 1992.

• LÓPEZ MORA, L. I. et al. Evaluación de la actividad antifúngica del quitosano en Alternaria alternata y en la calidad del mango ‘Tommy Atkins’ durante el almacenamiento. Revista Chapingo Serie Horticultura, 19(3): 315-331, 2013.

• MOHAMMADI, M. y KAZEMI, H. Changes in peroxidase and polyphenol oxidase activities in susceptible and resistant wheat heads inoculated with Fusarum graminearum and induced resistance. Plant Science, 162(4): 491-498, 2002.

• TERRY, L. A. y JOYCE, D. C. Elicitors of induced disease resistance in postharvest horticultural crops: a brief review. Postharvest Biology and Technology, 32(1): 1-13, 2004.

• TIAN, S. et al. Induction of defense responses against Alternaria rot by different elicitors in harvested pear fruit. Applied Microbiology and Biotechnology, 70(6): 729-734, 2006.

• ZHU, S. J. y MA, B. C. Benzothiadiazole or methyl jasmonateinduced resistance to Colletotrichum musae in harvested banana fruit is related to elevated defense enzyme activities. Journal Horticultural Science and Biotechnology, 82(4): 500-506, 2007.

Published

2015-12-31

Issue

Section

Artículos de Investigación

Categories