Molecular identification of isolates of Trichoderma spp. and promoting activity in Solanum lycopersicum L.
DOI:
https://doi.org/10.33064/iycuaa201771335Keywords:
Trichoderma ghanense, Trichoderma citrinoviride, Trichoderma harzianum, biofertilizer, saprophytic, seedlingsAbstract
The objective of this study was to isolate, identify and estimate the promoting plant growth of species of Trichoderma. Four strains were isolated, two were identified as T. ghanense (Th26-52 and Th02-04) and as T. citrinoviride (Th33-58) and once as T. harzianum (Th33-59). Promotion results in seedlings of Solanum lycopersicum L. cv. Rio Grande at concentrations of 106 conidios·ml-1 and 50% chemical fertilizer showed significant differences between treatments (p ≤ .01). Treatment with Trichoderma spp. increased from 24.28 to 38.53% the seedling height, 2.29 to 14.09% the stem diameter, 8.48 to 16.54% the root length, 0.37 to 2.68% the root volume and 0 to 23.07% the total dry biomass compared to the control with chemical fertilization 50%. T. ghanense (Th26-52) showed potential to improve seedling growth in S. lycopersicum.
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Bills, G. F., Christensen, M., Powell, M., & Thorn, G. (2004).
Saprobic soil fungi. En G. Mueller, F. Bills, & M. Foster (Eds.),
Biodiversity of fungi: Inventory and monitoring methods (pp.
-302). Amsterdam, Netherlands: Academic Press.
Bissett, J., Gams, W., Jaklitsch, W., & Samuels, G. J. (2015).
Accepted Trichoderma names in the year 2015. International
Mycological Association, 6(2), 263-295. doi:10.5598/
imafungus.2015.06.02.02
Cai, F., Chen, W., Wei, Z., Pang, G., Li, R., Ran, W., & Shen, Q.
(2015). Colonization of Trichoderma harzianum strain SQR-T037
on tomato roots and its relationship to plant growth, nutrient
availability and soil microflora. Plant and Soil, 388(1), 337-350.
doi:10.1007/s11104-014-2326-z
Candelero, D. J., Cristóbal, A. J., Reyes, R. A., Tun, S. J. M.,
Gamboa, A. M. M., & Ruíz, S. E. (2015). Trichoderma spp.
promotoras del crecimiento en plántulas de Capsicum
chinense Jacq. y antagónicas contra Meloidogyne incognita.
Revista Internacional de Botánica Experimental ɸYTON, 84,
-119. Recuperado de http://www.revistaphyton.fundromuloraggio.
org.ar/vol84-1/Candelero.pdf
Contreras-Cornejo, H. A., Macías-Rodríguez, L., Beltrán-Peña,
E., Herrera-Estrella, A., & López-Bucio, J. (2011). Trichodermainduced
plant immunity likely involves both hormonal- and
camalexin-dependent mechanisms in Arabidopsis thaliana
and confers resistance against necrotrophic fungi Botrytis
cinerea. Plant Signaling & Behavior, 6(10), 1554-1563.
doi:10.4161/psb.6.10.17443
Cubillos-Hinojosa, J., Valero, N., & Mejía, L. (2009). Trichoderma
harzianum como promotor del crecimiento vegetal del
maracuyá (Passiflora edulis var. flavicarpa Degener).
Agronomía Colombiana, 27(1), 81-86. Recuperado de http://
www.redalyc.org/articulo.oa?id=180314730011
Donoso, E., Lobos, G. A., & Rojas, N. (2008). Efecto de Trichoderma
harzianum y compost sobre el crecimiento de plántulas de Pinus
radiata en vivero. Bosque, 29(1), 52-57. Recuperado de http://www.
scielo.cl/pdf/bosque/v29n1/art06.pdf
Hermosa, R., Rubio, M. B., Cardoza, R. E., Nicolás, C., Monte,
E., & Gutiérrez, S. (2013). The contribution of Trichoderma to
balancing the costs of plant growth and defense. International
Microbiology, 16, 69-80. doi:10.2436/20.1501.01.181
Jaklitsch, W. M., & Voglmayr, H. (2014). New combinations in
Trichoderma (Hypocreaceae, Hypocreales). Mycotaxon, 126,
-156. doi:10.5248/126.143.
Kumar Solanki, M., Singh, N., Kumar Singh, R., Singh, P.,
Srivastava, A. K., Kumar, S., … Arora, D. K. (2011). Plant defense
activation and management of tomato root rot by a chitinfortified
Trichoderma/Hypocrea formulation. Phytoparasitica,
, 471-481. doi:10.1007/s12600-011-0188-y
Leelavathi, M. S, Vani, L., & Reena, P. (2014). Antimicrobial
activity of Trichoderma harzianum against bacteria and fungi.
International Journal of Current Microbiology and Applied
Sciences, 3(1), 96-103. Recuperado de http://www.ijcmas.
com/vol-3-1/M.S.Leelavathi,%20et%20al.pdf
López-Bucio, J., Pelagio-Flores, R., & Herrera-Estrella, A.
(2015). Trichoderma as biostimulant: exploiting the multilevel
properties of a plant beneficial fungus. Scientia Horticulturae,
, 109-123. Recuperado de http://dx.doi.org/10.1016/j.
scienta.2015.08.043
Marín-Guirao, J. I., Rodríguez-Romera, P., Lupión-Rodríguez,
B., Camacho-Ferre, F., & Tello-Marquina, J. C. (2016). Effect
of Trichoderma on horticultural seedlings’ growth promotion
depending on inoculum and substrate type. Journal of Applied
Microbiology, 121(4), 1095-1102. doi:10.1111/jam.13245
Martínez-Medina A., Alguacil, M. M., Pascual, J. A., & Van Wees,
S. C. (2014). Phytohormone profiles induced by Trichoderma
isolates correspond with their biocontrol and plant growthpromoting
activity on melon plants. Journal of Chemical
Ecology, 40(7), 804-815. doi:10.1007/s10886-014-0478-1
Moo-Koh, F. A., Alejo, J. C., Reyes-Ramírez, A., Tun-Suárez, J. M.,
Sandoval-Luna, R., & Ramírez-Pool, J. A. (2014). Actividad in
vitro del extracto acuoso de Bonellia flammea contra hongos
fitopatógenos. Agrociencia, 48(8), 833-845. Recuperado de
http://www.scielo.org.mx/pdf/agro/v48n8/v48n8a6.pdf
National Center for Biotechnology Information. Basic Local
Alignment Search Too (BLAST). Recuperado de https://www.
ncbi.nlm.nih.gov/
Pavone Maniscalco, D., & Dorta, B. (2015). Diversidad
del hongo Trichoderma spp. en plantaciones de maíz de
Venezuela. Asociación Interciencia, 40(1), 23-31. Recuperado
de http://www.redalyc.org/articulo.oa?id=33933115004
Tamura, K., Stecher, G., Peterson, D., Filipski, A., & Kumar, S.
(2013). MEGA6: Molecular Evolutionary Genetics Analysis
version 6.0. Molecular Biology and Evolution, 30(12), 2725-2729.
doi:10.1093/molbev/mst197
Vinale, F., Nigro, M., Sivasithamparam, K., Flematti, G.,
Ghisalberti, E. L., Ruocco, M., … Lorito, M. (2013). Harzianic
acid: a novel siderophore from Trichoderma harzianum. FEMS
Microbiol Lett, 347(2), 123-129. doi:10.1111/1574-6968.12231
White, T. J., Bruns, T., Lee, S., & Taylor, J. (1990). Amplification
and direct sequencing of fungal ribosomal RNA genes for
phylogenetics. En M. A. Inns, D. H. Gelfand, J. J. Sninsky,
& T. J. White (Eds.), PCR Protocols: A guide to methods and
applications (pp. 315-322). San Diego, CA: Academic Press.
Zhenyu, S., Man, X., Xinzheng, G., Libo, T., & Li, L. (2014). Primary
study on biological control potential of Trichoderma harzianum
TL-1. Plant Diseases and Pests, 5(2), 25-28. Recuperado de
http://search.proquest.com/openview/d997794458cd80f3865
bd15fb5335546/1?pq-origsite=gscholar
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