Molecular identification of isolates of Trichoderma spp. and promoting activity in Solanum lycopersicum L.

Authors

  • Felicia Amalia Moo-Koh Instituto Tecnológico de Conkal
  • Jairo Cristóbal Alejo Instituto Tecnológico de Conkal
  • Arturo Reyes Ramírez Instituto Tecnológico de Conkal
  • José María Tun Suárez Instituto Tecnológico de Conkal
  • Marcela Gamboa Angulo Centro de Investigación Científica de Yucatán

DOI:

https://doi.org/10.33064/iycuaa201771335

Keywords:

Trichoderma ghanense, Trichoderma citrinoviride, Trichoderma harzianum, biofertilizer, saprophytic, seedlings

Abstract

The objective of this study was to isolate, identify and estimate the promoting plant growth of species of Trichoderma. Four strains were isolated, two were identified as T. ghanense (Th26-52 and Th02-04) and as T. citrinoviride (Th33-58) and once as T. harzianum (Th33-59). Promotion results in seedlings of Solanum lycopersicum L. cv. Rio Grande at concentrations of 106 conidios·ml-1 and 50% chemical fertilizer showed significant differences between treatments (p ≤ .01). Treatment with Trichoderma spp. increased from 24.28 to 38.53% the seedling height, 2.29 to 14.09% the stem diameter, 8.48 to 16.54% the root length, 0.37 to 2.68% the root volume and 0 to 23.07% the total dry biomass compared to the control with chemical fertilization 50%. T. ghanense (Th26-52) showed potential to improve seedling growth in S. lycopersicum.

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Author Biographies

Felicia Amalia Moo-Koh, Instituto Tecnológico de Conkal

División de Estudios de Posgrado e Investigación, Instituto Tecnológico de
Conkal. Avenida Tecnológico s/n, C. P. 97345, Conkal, Yucatán, México.

Jairo Cristóbal Alejo, Instituto Tecnológico de Conkal

División de Estudios de Posgrado e Investigación, Instituto Tecnológico de
Conkal. Avenida Tecnológico s/n, C. P. 97345, Conkal, Yucatán, México.

Arturo Reyes Ramírez, Instituto Tecnológico de Conkal

División de Estudios de Posgrado e Investigación, Instituto Tecnológico de
Conkal. Avenida Tecnológico s/n, C. P. 97345, Conkal, Yucatán, México.

José María Tun Suárez, Instituto Tecnológico de Conkal

División de Estudios de Posgrado e Investigación, Instituto Tecnológico de
Conkal. Avenida Tecnológico s/n, C. P. 97345, Conkal, Yucatán, México.

Marcela Gamboa Angulo, Centro de Investigación Científica de Yucatán

Unidad de Biotecnología, Centro de Investigación Científica de Yucatán,
A. C. Calle 43 No. 130, Colonia Chuburná de Hidalgo, C. P. 97205, Mérida,
Yucatán, México

References

Bills, G. F., Christensen, M., Powell, M., & Thorn, G. (2004).

Saprobic soil fungi. En G. Mueller, F. Bills, & M. Foster (Eds.),

Biodiversity of fungi: Inventory and monitoring methods (pp.

-302). Amsterdam, Netherlands: Academic Press.

Bissett, J., Gams, W., Jaklitsch, W., & Samuels, G. J. (2015).

Accepted Trichoderma names in the year 2015. International

Mycological Association, 6(2), 263-295. doi:10.5598/

imafungus.2015.06.02.02

Cai, F., Chen, W., Wei, Z., Pang, G., Li, R., Ran, W., & Shen, Q.

(2015). Colonization of Trichoderma harzianum strain SQR-T037

on tomato roots and its relationship to plant growth, nutrient

availability and soil microflora. Plant and Soil, 388(1), 337-350.

doi:10.1007/s11104-014-2326-z

Candelero, D. J., Cristóbal, A. J., Reyes, R. A., Tun, S. J. M.,

Gamboa, A. M. M., & Ruíz, S. E. (2015). Trichoderma spp.

promotoras del crecimiento en plántulas de Capsicum

chinense Jacq. y antagónicas contra Meloidogyne incognita.

Revista Internacional de Botánica Experimental ɸYTON, 84,

-119. Recuperado de http://www.revistaphyton.fundromuloraggio.

org.ar/vol84-1/Candelero.pdf

Contreras-Cornejo, H. A., Macías-Rodríguez, L., Beltrán-Peña,

E., Herrera-Estrella, A., & López-Bucio, J. (2011). Trichodermainduced

plant immunity likely involves both hormonal- and

camalexin-dependent mechanisms in Arabidopsis thaliana

and confers resistance against necrotrophic fungi Botrytis

cinerea. Plant Signaling & Behavior, 6(10), 1554-1563.

doi:10.4161/psb.6.10.17443

Cubillos-Hinojosa, J., Valero, N., & Mejía, L. (2009). Trichoderma

harzianum como promotor del crecimiento vegetal del

maracuyá (Passiflora edulis var. flavicarpa Degener).

Agronomía Colombiana, 27(1), 81-86. Recuperado de http://

www.redalyc.org/articulo.oa?id=180314730011

Donoso, E., Lobos, G. A., & Rojas, N. (2008). Efecto de Trichoderma

harzianum y compost sobre el crecimiento de plántulas de Pinus

radiata en vivero. Bosque, 29(1), 52-57. Recuperado de http://www.

scielo.cl/pdf/bosque/v29n1/art06.pdf

Hermosa, R., Rubio, M. B., Cardoza, R. E., Nicolás, C., Monte,

E., & Gutiérrez, S. (2013). The contribution of Trichoderma to

balancing the costs of plant growth and defense. International

Microbiology, 16, 69-80. doi:10.2436/20.1501.01.181

Jaklitsch, W. M., & Voglmayr, H. (2014). New combinations in

Trichoderma (Hypocreaceae, Hypocreales). Mycotaxon, 126,

-156. doi:10.5248/126.143.

Kumar Solanki, M., Singh, N., Kumar Singh, R., Singh, P.,

Srivastava, A. K., Kumar, S., … Arora, D. K. (2011). Plant defense

activation and management of tomato root rot by a chitinfortified

Trichoderma/Hypocrea formulation. Phytoparasitica,

, 471-481. doi:10.1007/s12600-011-0188-y

Leelavathi, M. S, Vani, L., & Reena, P. (2014). Antimicrobial

activity of Trichoderma harzianum against bacteria and fungi.

International Journal of Current Microbiology and Applied

Sciences, 3(1), 96-103. Recuperado de http://www.ijcmas.

com/vol-3-1/M.S.Leelavathi,%20et%20al.pdf

López-Bucio, J., Pelagio-Flores, R., & Herrera-Estrella, A.

(2015). Trichoderma as biostimulant: exploiting the multilevel

properties of a plant beneficial fungus. Scientia Horticulturae,

, 109-123. Recuperado de http://dx.doi.org/10.1016/j.

scienta.2015.08.043

Marín-Guirao, J. I., Rodríguez-Romera, P., Lupión-Rodríguez,

B., Camacho-Ferre, F., & Tello-Marquina, J. C. (2016). Effect

of Trichoderma on horticultural seedlings’ growth promotion

depending on inoculum and substrate type. Journal of Applied

Microbiology, 121(4), 1095-1102. doi:10.1111/jam.13245

Martínez-Medina A., Alguacil, M. M., Pascual, J. A., & Van Wees,

S. C. (2014). Phytohormone profiles induced by Trichoderma

isolates correspond with their biocontrol and plant growthpromoting

activity on melon plants. Journal of Chemical

Ecology, 40(7), 804-815. doi:10.1007/s10886-014-0478-1

Moo-Koh, F. A., Alejo, J. C., Reyes-Ramírez, A., Tun-Suárez, J. M.,

Sandoval-Luna, R., & Ramírez-Pool, J. A. (2014). Actividad in

vitro del extracto acuoso de Bonellia flammea contra hongos

fitopatógenos. Agrociencia, 48(8), 833-845. Recuperado de

http://www.scielo.org.mx/pdf/agro/v48n8/v48n8a6.pdf

National Center for Biotechnology Information. Basic Local

Alignment Search Too (BLAST). Recuperado de https://www.

ncbi.nlm.nih.gov/

Pavone Maniscalco, D., & Dorta, B. (2015). Diversidad

del hongo Trichoderma spp. en plantaciones de maíz de

Venezuela. Asociación Interciencia, 40(1), 23-31. Recuperado

de http://www.redalyc.org/articulo.oa?id=33933115004

Tamura, K., Stecher, G., Peterson, D., Filipski, A., & Kumar, S.

(2013). MEGA6: Molecular Evolutionary Genetics Analysis

version 6.0. Molecular Biology and Evolution, 30(12), 2725-2729.

doi:10.1093/molbev/mst197

Vinale, F., Nigro, M., Sivasithamparam, K., Flematti, G.,

Ghisalberti, E. L., Ruocco, M., … Lorito, M. (2013). Harzianic

acid: a novel siderophore from Trichoderma harzianum. FEMS

Microbiol Lett, 347(2), 123-129. doi:10.1111/1574-6968.12231

White, T. J., Bruns, T., Lee, S., & Taylor, J. (1990). Amplification

and direct sequencing of fungal ribosomal RNA genes for

phylogenetics. En M. A. Inns, D. H. Gelfand, J. J. Sninsky,

& T. J. White (Eds.), PCR Protocols: A guide to methods and

applications (pp. 315-322). San Diego, CA: Academic Press.

Zhenyu, S., Man, X., Xinzheng, G., Libo, T., & Li, L. (2014). Primary

study on biological control potential of Trichoderma harzianum

TL-1. Plant Diseases and Pests, 5(2), 25-28. Recuperado de

http://search.proquest.com/openview/d997794458cd80f3865

bd15fb5335546/1?pq-origsite=gscholar

Published

2017-08-31

How to Cite

Moo-Koh, F. A., Cristóbal Alejo, J., Reyes Ramírez, A., Tun Suárez, J. M., & Gamboa Angulo, M. (2017). Molecular identification of isolates of Trichoderma spp. and promoting activity in Solanum lycopersicum L. Investigación Y Ciencia De La Universidad Autónoma De Aguascalientes, (71), 5–11. https://doi.org/10.33064/iycuaa201771335

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