Kinetic growth of Fusarium oxysporum cultured on different glucose and pectin levels
DOI:
https://doi.org/10.33064/iycuaa2016672272Keywords:
depolymerization, F. oxysporum, glucose, pectic enzymes, pectin, invasion rateAbstract
Pectin and pectic enzymes are essential in the food industry, mainly in the juice industry, confectionary and bakery. Diverse fungal species are able to produce pectinases in order to degrade pectin for surviving and growth. In the present study, radial growth rate of Fusarium oxysporum was evaluated. Glucose, pectin and pectin hydrolysates (with different degrees of depolymerization) were employed as substrates. Obtained results suggest that low substrate concentrations promoted a higher invasion rate, whereas, under high substrate concentrations it was lower. Control of the phenomenon of catabolic repression through definition of initial concentrations of both substrates, is essential for pectinases production under solid state fermentations and to increase biotechnology applications of these enzymes.
Downloads
Download data is not yet available.
Metrics
Metrics Loading ...
References
• Barragán , J. C. A. et al. Efecto de la temperatura y pH sobre la actividad y estabilidad de pectinasas producidas por Bacillus spp. Revista de Investigación Científica (REBIOL), 34(1): 33-41, 2014.
• Di Pietro , A. y Roncero , M. I. G. Purification and characterization of an exo-polygalacturonase from the tomato vascular wilt pathogen Fusarium oxysporum f.sp. lycopersici. FEMS Microbiology Letters, 145(2): 295-299, 1996.
• Gutiérrez Sánchez , G. et al. Comparación de dos métodos para la selección de cepas para su uso en fermentación en medio sólido: Crecimiento radial y longitudinal. Memorias VIII Congreso Nacional de Biotecnología y Bioingeniería, p. 104, 1999.
• Izquier Do, J. F. et al. Cinética de las reacciones químicas. Metodología Series: 16. Barcelona, España: Edicions Universitat de Barcelona, p. 288, 2004.
• Larcher , L. I. et al. Simulación de crecimiento de microorganismos bajo distintas condiciones de movilidad. Mecánica Computacional, XXVII, 3381-3395, 2008.
• Mejía GIRALDO, L. F. et al. Aprovechamiento del residuo agroindustrial del mango común (Mangifera indica L.) en la obtención de azúcares fermentables. Ingeniería y Ciencia, 3(6): 41-62, 2007.
• Nelson , N. A photometric adaptation of the Somogyi method for determination of glucose. Journal of Biological Chemistry, 153, 375-380, 1944.
• Niture , S. K. et al. Inactivation of polygalacturonase and pectate lyase produced by pH tolerant fungus Fusarium moniliforme NCIM 1276 in a liquid medium and in the host tissue. Microbiological Research, 163(1): 51-62, 2008.
• Orz úa, G. Aprovechamiento de residuos agroindustriales como soporte de crecimiento fúngico para la fermentación en medio sólido. Tesis de licenciatura. Saltillo, Coahuila, México: Universidad Autónoma de Coahuila, 2003.
• Sánchez Aldana Villarruel , D. et al. Moléculas pécticas: extracción y su potencial aplicación como empaque. Tecnociencia, V(2): 76-82, 2011.
• Somogyi , M. Notes on sugar determination. Journal of Biological Chemistry, 195(1): 19-23, 1952.
• SuÁrez -Segundo , J. L. et al. Growth of colonies and hyphal ultraestructure of filamentous fungi grown on dibutyl phthalate and di (2-ethylhexyl) phthalate. Revista Mexicana de Ingeniería Química, 12(3): 499-504, 2013.
• Viniegra González , G. y Favela Torres , E. Why Solid State Fermentation Seems to be Resistan to Catabolite Repression? Food Technology and Biotechnology, 44(3): 397-406, 2006.
• ANDRE LEROUX, G. et al. Endopolygalacturonases reveal molecular features for processivity pattern and tolerance towards acetylated pectin. Biochimica et. Biophysica Acta, 1794(1): 5-13, 2009. doi: 10.1016/j.bbapap.2008.09.004.
• BENEDETTI, M. et al. A single amino-acid substitution allows endo-polygalacturonase of Fusarium verticillioides to acquire recognition by PGIP2 from Phaseolus vulgaris. PLoS ONE, 8(11): e80610, 2013. doi:10.1371/journal.pone.0080610.
• KANT, S. et al. Purification and physicochemical properties of polygalacturonase from Aspergillus niger MTCC 3323. Protein Expression and Purification, 87(1): 11-16, 2014. doi: 10.1016/j.pep.2012.09.014.
• MARTINS, S. et al. Bioactive phenolic compounds: Production and extraction by solid-state fermentation. A review. Biotechnology Advances, 29(3): 365-373, 2011. doi: 10.1016/j.biotechadv.2011.01.008.
• MONOD, J. The growth of bacterial cultures. Annual Review of Microbiology, 3, 371-394, 1949. doi:10.1146/annurev.mi.03.100149.002103.
• ORZÚA, M. C. et al. Exploitation of agro industrial wastes as immobilization carrier for solid–state fermentation. Industrial Crops and Products, 30(1): 24-27, 2009. doi: 10.1016/j.indcrop.2009.02.001.
• RANGEL RODRÍGUEZ, A. M. et al. Immobilization of Pectinesterase in Genipin-Crosslinked Chitosan Membrane for Low Methoxyl Pectin Production. Applied Biochemistry and Biotechnology, 174(8): 2941-2950, 2014. doi: 10.1007/s12010-014-1238-y.
• WU, H. S. et al. Cinnamic acid inhibits growth but stimulates production of pathogenesis factors by in vitro cultures of Fusarium oxysporum f.sp. niveum. Journal of Agricultural and Food Chemistry, 56(4): 1316-1321, 2008. doi: 10.1021/jf0726482.
• Di Pietro , A. y Roncero , M. I. G. Purification and characterization of an exo-polygalacturonase from the tomato vascular wilt pathogen Fusarium oxysporum f.sp. lycopersici. FEMS Microbiology Letters, 145(2): 295-299, 1996.
• Gutiérrez Sánchez , G. et al. Comparación de dos métodos para la selección de cepas para su uso en fermentación en medio sólido: Crecimiento radial y longitudinal. Memorias VIII Congreso Nacional de Biotecnología y Bioingeniería, p. 104, 1999.
• Izquier Do, J. F. et al. Cinética de las reacciones químicas. Metodología Series: 16. Barcelona, España: Edicions Universitat de Barcelona, p. 288, 2004.
• Larcher , L. I. et al. Simulación de crecimiento de microorganismos bajo distintas condiciones de movilidad. Mecánica Computacional, XXVII, 3381-3395, 2008.
• Mejía GIRALDO, L. F. et al. Aprovechamiento del residuo agroindustrial del mango común (Mangifera indica L.) en la obtención de azúcares fermentables. Ingeniería y Ciencia, 3(6): 41-62, 2007.
• Nelson , N. A photometric adaptation of the Somogyi method for determination of glucose. Journal of Biological Chemistry, 153, 375-380, 1944.
• Niture , S. K. et al. Inactivation of polygalacturonase and pectate lyase produced by pH tolerant fungus Fusarium moniliforme NCIM 1276 in a liquid medium and in the host tissue. Microbiological Research, 163(1): 51-62, 2008.
• Orz úa, G. Aprovechamiento de residuos agroindustriales como soporte de crecimiento fúngico para la fermentación en medio sólido. Tesis de licenciatura. Saltillo, Coahuila, México: Universidad Autónoma de Coahuila, 2003.
• Sánchez Aldana Villarruel , D. et al. Moléculas pécticas: extracción y su potencial aplicación como empaque. Tecnociencia, V(2): 76-82, 2011.
• Somogyi , M. Notes on sugar determination. Journal of Biological Chemistry, 195(1): 19-23, 1952.
• SuÁrez -Segundo , J. L. et al. Growth of colonies and hyphal ultraestructure of filamentous fungi grown on dibutyl phthalate and di (2-ethylhexyl) phthalate. Revista Mexicana de Ingeniería Química, 12(3): 499-504, 2013.
• Viniegra González , G. y Favela Torres , E. Why Solid State Fermentation Seems to be Resistan to Catabolite Repression? Food Technology and Biotechnology, 44(3): 397-406, 2006.
• ANDRE LEROUX, G. et al. Endopolygalacturonases reveal molecular features for processivity pattern and tolerance towards acetylated pectin. Biochimica et. Biophysica Acta, 1794(1): 5-13, 2009. doi: 10.1016/j.bbapap.2008.09.004.
• BENEDETTI, M. et al. A single amino-acid substitution allows endo-polygalacturonase of Fusarium verticillioides to acquire recognition by PGIP2 from Phaseolus vulgaris. PLoS ONE, 8(11): e80610, 2013. doi:10.1371/journal.pone.0080610.
• KANT, S. et al. Purification and physicochemical properties of polygalacturonase from Aspergillus niger MTCC 3323. Protein Expression and Purification, 87(1): 11-16, 2014. doi: 10.1016/j.pep.2012.09.014.
• MARTINS, S. et al. Bioactive phenolic compounds: Production and extraction by solid-state fermentation. A review. Biotechnology Advances, 29(3): 365-373, 2011. doi: 10.1016/j.biotechadv.2011.01.008.
• MONOD, J. The growth of bacterial cultures. Annual Review of Microbiology, 3, 371-394, 1949. doi:10.1146/annurev.mi.03.100149.002103.
• ORZÚA, M. C. et al. Exploitation of agro industrial wastes as immobilization carrier for solid–state fermentation. Industrial Crops and Products, 30(1): 24-27, 2009. doi: 10.1016/j.indcrop.2009.02.001.
• RANGEL RODRÍGUEZ, A. M. et al. Immobilization of Pectinesterase in Genipin-Crosslinked Chitosan Membrane for Low Methoxyl Pectin Production. Applied Biochemistry and Biotechnology, 174(8): 2941-2950, 2014. doi: 10.1007/s12010-014-1238-y.
• WU, H. S. et al. Cinnamic acid inhibits growth but stimulates production of pathogenesis factors by in vitro cultures of Fusarium oxysporum f.sp. niveum. Journal of Agricultural and Food Chemistry, 56(4): 1316-1321, 2008. doi: 10.1021/jf0726482.
Downloads
Published
2016-04-30
License
Las obras publicadas en versión electrónica de la revista están bajo la licencia Creative Commons Atribución-NoComercial-CompartirIgual 4.0 Internacional (CC BY-NC-SA 4.0)
