Effect of cryoprotectants on the morphology and electrolyte leakage on axillary buds of cryopreserved grapevine cv. `Flame Seedless´
DOI:
https://doi.org/10.33064/iycuaa201772220Keywords:
Vitis vinifera L., PVS2, PVS3, glycerol, viability, vitrificationAbstract
The cryopreservation has revolutionized the field of biotechnology. Frozen in liquid nitrogen (LN) preserves long living cells. In this sense, in this paper were evaluated three conditions of cryopreservation based on vitrification of buds of grapevine. The buds were subjected to the PVS2, PVS3 and glycerol for 0-420 min, and submerged in LN for one hour. After the incubation time electrolyte leakage was determined as a viability measurement, and tissue damage was evaluated through stereoscopic observation. Based on the viability percentage the best preservation method was using PVS3 solution (30%) followed by glycerol (25%) and PVS2 (<10%). The images of the buds exposed to PVS3 shows no tissue damage unlike to PVS2 and glycerol, were not sufficient to preserve buds tissue.
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