Effect of cryoprotectants on the morphology and electrolyte leakage on axillary buds of cryopreserved grapevine cv. `Flame Seedless´
DOI:
https://doi.org/10.33064/iycuaa201772220Keywords:
Vitis vinifera L., PVS2, PVS3, glycerol, viability, vitrificationAbstract
The cryopreservation has revolutionized the field of biotechnology. Frozen in liquid nitrogen (LN) preserves long living cells. In this sense, in this paper were evaluated three conditions of cryopreservation based on vitrification of buds of grapevine. The buds were subjected to the PVS2, PVS3 and glycerol for 0-420 min, and submerged in LN for one hour. After the incubation time electrolyte leakage was determined as a viability measurement, and tissue damage was evaluated through stereoscopic observation. Based on the viability percentage the best preservation method was using PVS3 solution (30%) followed by glycerol (25%) and PVS2 (<10%). The images of the buds exposed to PVS3 shows no tissue damage unlike to PVS2 and glycerol, were not sufficient to preserve buds tissue.
Downloads
Metrics
References
Al-Zoubi, O. M., & Normah, M .N. (2015). Ultrastructural response of embryonic axes of Fortunella polyandra to dehydration and cryopreservation. Cryo-Letters, 36(6), 379-391. Recuperado de http://www.ingentaconnect.com/content/cryo/cryo/2015/00000036/00000006/art00004
Bayati, S., Shams-Bakhsh, M., & Moini, A. (2011). Elimination of Grapevine virus A (GVA) by cryotherapy and electrotherapy. En Journal of Agricultural Science and Technology, 13(3), 443-450. Recuperado de http://journals.modares.ac.ir/article_4713_c51d0b2176daee020a071e9d33f2aa51.pdf
Ben-Amar, A., Daldoul, S., Allel, D., Reustle, G., & Mliki, A. (2013). Reliable encapsulation-based cryopreservation protocol for safe storage and recovery of grapevine embryogenic cell cultures. En Scientia Horticulturae, 157, 32-38. doi:10.1016/j.scienta.2013.04.005
Benson, E. E. (2008). Cryopreservation Theory. En B. M. Reed (Ed.), Plant Cryopreservation: A Practical Guide (pp. 15-30). New York, NY: Springer.
Bhattacharya, S., & Prajapati, B. G. (2016). A review on cryoprotectant and its modern implication in cryonics. Asian Journal of Pharmaceutics, 10(3), 154-159. doi: 10.22377/ajp.v10i3.721
Chalker-Scott, L., Fuchigami, L. H., & Harber, R. M. (1989). Spectrophotometric measurement of leached phenolic compounds as an indicator of freeze damage. Journal of the American Society for Horticultural Science, 114, 315-319. Recuperado de https://ir.library.oregonstate.edu/xmlui/bitstream/handle/1957/25663/CHALKERSCOTTLINDA1989.pdf?sequence=1#page=74
Engelmann, F. (2012). Germplasm collection, storage and preservation. En A. Altman & P. M. Hazegawa (Eds.), Plant biotechnology and agriculture-Prospects for the 21st Century (pp. 255-268). Oxford, UK: Academic Press.
Ganino, T., Silvanini, A., Beghé, D., Benelli, C., Lambardi, M., & Fabbri, A. (2012). Anatomy and osmotic potential of the Vitis rootstock shoot tips recalcitrant to cryopreservation. Biologia Plantarum, 56(1), 78-82. doi: https://doi.org/10.1007/s10535-012-0019-0
González-Arnao, M. T., Panta, A., Roca, W. M., Escobar, R. H., & Engelmann, F. (2008). Development and large scale application of cryopreservation techniques for shoot and somatic embryo cultures of tropical crops. Plant Cell Tissue and Organ Culture, 92(1), 1-13. doi: 10.1007/s11240-007-9303-7
González-Benito, M., Martín, C., & Vidal, J. (2009). Cryopreservation of embryogenic cell suspensions of the Spanish grapevine cultivars ‘Albariño’ and ‘Tempranillo’. Vitis, 48(3), 131-136. Recuperado de http://www.vitis-vea.de/admin/volltext/w1%2009%201298.pdf
Kaczmarczyk, A., Menon, A., Al-Hanbali, A., Funnekotter, B., Bunn, E., Phang, P. Y., & Mancera, R. L. (2012). Current issues in plant cryopreservation. En I. Katkov (Ed.), Current frontiers in cryobiology (592 pp.). Rijeka, Croatia: InTech Open Access Publisher. doi: 10.5772/32860
Kaviani, B. (2011). Conservation of plant genetic resources by cryopreservation. Australian Journal of Crop Science, 5(6), 778-800. Recuperado de http://www.cropj.com/kaviani_5_6_2011_778_800.pdf
Lazo-Javalera, M. F., Troncoso-Rojas, R., Tiznado-Hernández, M. E., Martínez-Téllez, M. A., Vargas-Arispuro, I., Islas-Osuna, M. A., & Rivera-Domínguez, M. (2016). Surface disinfection procedure and in vitro regeneration of grapevine (Vitis vinifera L.) axillary buds. SpringerPlus, 5, 453. doi: 10.1186/s40064-016-2081-0
Lin, L., Yuan, B., Wang, D., & Li, W. (2014). Cryopreservation of adventitious shoot tips of Paraisometrum Mileense by droplet vitrification. En CryoLetters, 35(1), 22-28. Recuperado de http://www.ingentaconnect.com/content/cryo/cryo/2014/00000035/00000001/art00004
Liu, G. T., Wang, J. F., Cramer, G., Dai, Z. W., Duan, W., Xu, H. G.,…, Li, S. H. (2012). Transcriptomic analysis of grape (Vitis vinifera L.) leaves during and after recovery from heat stress. BMC Plant Biology, 12, 174. doi:10.1186/1471-2229-12-174
Makowska, Z., Keller, J., & Engelmann, F. (1999). Cryopreservation of apices isolated from garlic (Allium sativum L.) bulbils and cloves. En CryoLetters, 20(3), 175-182. Recuperado de http://www.documentation.ird.fr/hor/fdi:010018253
Marković, Z., Chatelet, P., Sylvestre, I., Kontić, J., & Engelmann, F. (2013). Cryopreservation of grapevine (Vitis vinifera L.) in vitro shoot tips. Open Life Sciences, 8(10), 993-1000. doi: 10.2478/s11535-013-0223-8
Martínez-Montero, M. E., Martínez, J., & Engelmann, F. (2008). Cryopreservation of sugarcane somatic embryos. CryoLetters, 29(3), 229-242. Recuperado de http://www.ingentaconnect. com/content/cryo/cryo/2008/00000029/00000003/art00006
Matsumoto, T., & Sakai, A. (2003). Cryopreservation of axillary shoot tips of in vitro-grown grape (Vitis) by a twostep vitrification protocol. Euphytica, 131(3), 299-304. doi: 10.1023/A:1024024909864
Mazur, P. (1960). Physical factors implicated in the death of microorganisms at subzero temperatures. Annals of the New York Academy of Sciences, 85, 610-629. doi: 10.1111/j.1749-6632.1960.tb49986.x
Murashige, T., & Skoog, F. (1962). A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiologia Plantarum, 15, 473-497. doi: 10.1111/j.1399-3054.1962.tb08052.x
Nishizawa, S., Sakai, A., Amano, Y., & Matsuzawa, T. (1993). Cryopreservation of asparagus (Asparagus-Officinalis L.) embryogenic suspension cells and subsequent plant regeneration by vitrification. Plant Science, 91(1), 67-73. doi: 10.1016/0168-9452(93)90189-7
Pathirana, R., Mclachlan, A., Hedderley, D., Panis, B., & Carimi, F. (2016). Pre-treatment with salicylic acid improves plant regeneration after cryopreservation of grapevine (Vitis spp.) by droplet vitrification. Acta Physiologiae Plantarum, 38, 12. doi: 10.1007/s11738-015-2026-1
Pereira, C. S., & Hünenberger, P. H. (2006). Interaction of the sugars trehalose, maltose and glucose with a phospholipid bilayer: a comparative molecular dynamics study. The Journal of Physical Chemistry B, 110(31), 15572-15581. doi: 10.1021/jp060789l
Pukacki, P. M., & Juszczyk, K. (2015). Desiccation sensitivity and cryopreservation of the embryogenic axes of the seeds of two Acer species. Trees, 29(2), 385-396. doi: 10.1007/s00468-014-1118-7
Sakai, A., & Engelmann, F. (2007). Vitrification, encapsulationvitrification
and droplet-vitrification: A review. CryoLetters, 28(3), 151-172. Recuperado de http://www.ingentaconnect. com/content/cryo/cryo/2007/00000028/00000003/art00002
Sakai, A., Kobayashi, S., & Oiyama, I. (1990). Cryopreservation of nucellar cells of navel orange (Citrus sinensis Osb. var. brasiliensis Tanaka) by vitrification. Plant Cell Reports, 9(1), 30-33. doi: 10.1007/BF00232130
Shatnawi, M. A. (2011). Cryopreservation of Capparis spinosa shoot tips via vitrification, encapsulation dehydration and encapsulation vitrification. World Applied Sciences Journal, 15(3), 318-325. Recuperado de https://pdfs.semanticscholar.org/3391/bbb8d5458f05f7a1a7fe3bc57002925c94b0.pdf
Teixeira, A. S., González-Benito, M. E., & Molina-García, A. D. (2013). Glassy state and cryopreservation of mint shoot tips. Biotechnology Progress, 29(3), 707-717. doi:10.1002/btpr.1711
Vasanth, K., & Vivier, M. A. (2011). Improved cryopreservation procedure for long term storage of synchronised culture of grapevine. Biologia Plantarum, 55(2), 365-369. doi: 10.1007/s10535-011-0056-0
Volk, G. M., Harris, J. L., & Rotindo, K. E. (2006). Survival of mint shoot tips after exposure to cryoprotectant solution components. Cryobiology, 52(2), 305-308. doi: 10.1016/j.cryobiol.2005.11.003
Wang, B., Li, J. W., Zhang, Z. B., Wang, R. R., Ma, Y. L., Blystad, D. R.,…, Wang, Q. C. (2014). Three vitrification-based cryopreservation procedures cause different cryo-injuries to potato shoot tips while all maintain genetic integrity in regenerants. Journal of Biotechnology, 184, 47-55. doi:
1016/j.jbiotec.2014.04.021
Zhai, Z., Wu, Y., Engelmann, F., Chen R., & Zhao, Y. (2003). Genetic stability assessments of plantlets regenerated from cryopreserved in vitro cultured grape and kiwi shoot-tips using rapd.
CryoLetters, 24(5), 315-322. Recuperado de http://www.ingentaconnect.
com/content/cryo/cryo/2003/00000024/00000005/art00006
Downloads
Published
How to Cite
License
Las obras publicadas en versión electrónica de la revista están bajo la licencia Creative Commons Atribución-NoComercial-CompartirIgual 4.0 Internacional (CC BY-NC-SA 4.0)
