Vibrio detection by amplification of pathogenicity genes in shrimp Litopenaeus vannamei cultured in a greenhouse system
DOI:
https://doi.org/10.33064/iycuaa201772218Keywords:
Vibrio spp, Vibrio harveyi, toxR gene, hemolysin gene, Litopenaeus vannameiAbstract
The infections by vibrio are an important problem in shrimp culture due to high mortalities and large economic losses caused. The aim of the study was to identify genes of pathogenicity in vibrio bacteria isolated of L. vannamei shrimp cultured in an intensive system on greenhouse with zero water exchange. It was determined total bacterial count and vibrio in hemolymph, hepatopancreas and gills of the shrimp. The strains were identified as Vibrio ssp., V. harveyi hemolysin gene (vhh), V. harveyi toxR and other pathogenic vibrio species by PCR. Both genes (hemolysin and toxR) were identified in four isolates of shrimp. This methodology could be a tool in the detection of V. harveyi for prevention and control. A highlight of this study was the absence of V. cholerae (V. chol), V. vulnificus (Vvh-785) y V. harveyi (LuxN)
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Arnold, S. J., Coman, F. E., Jackson, C. J., & Groves, S. A. (2009). High-intensity, zero water-exchange production of juvenile tiger shrimp, Penaeus monodon: An evaluation of artificial substrates and stocking density. Aquaculture, 293(1-2), 42-48.
Arzola G., J., Piña V., P., Nieves S., M., & Medina J., M. (2013). Supervivencia de postlarvas de camarón blanco Litopenaeus vannamei a diferentes salinidades y temperatura. Revista MVZ Córdova, 18(Supl.), 3618-3625.
Bermúdez-Almada, M. C., Espinosa-Plascencia, A., Santiago-Hernández, M. L., Barajas-Borgo, C. J., & Acedo-Félix, E. (2014). Comportamiento de oxitetraciclina en camarón de cultivo Litopenaeus vannamei y la sensibilidad a tres antibióticos de bacterias de Vibrio aisladas de los organismos. Biotecnia, 16(3), 29-37.
Boyd, C. E., Treece, G., Engle, R. C., Valderrama, D. V., Lightner, C. R., Pantoja, J., …, Benner, R. (2001). Consideraciones sobre la calidad del agua y del suelo en cultivos de camarón. En M. C. Haws, & C. E. Boyd (Ed.), Métodos para mejorar la camaronicultura en Centroamérica (pp. 1-30). Managua, Nicaragua: Editorial-Imprenta UCA.
Cohen, J. M., Samocha, T. M., Fox, J. M., Gandy, R. L., & Lawrence, A. L. (2005). Characterization of water quality factors during intensive raceway production of juvenile Litopenaeus vannamei using limited discharge and biosecure management tools. Aquacultural Engineering, 32(3-4), 425-442. Conejero, M. J., & Hedreyda, C. T. (2003). Isolation and partial toxR gene of Vibrio harveyi and design of toxR-targeted PCR primer for species detection. Journal of Applied Microbiology, 95(3), 602-611.
__________ (2004). PCR detection of hemolysin (vhh) gene in Vibrio harveyi. Journal of General Applied Microbiology, 50(3), 137-142.
Franco, P., & Hedreyda, C. (2006). Amplification and sequence analysis of the full length toxR gen in Vibrio harveyi. The Journal of General Applied Microbiology, 52(5), 281-287.
Frías-Espericueta, M. G., & Páez-Osuna, F. (2001). Toxicidad de los compuestos del nitrógeno en camarones. En F. Paéz-Osuna (Ed.), Camaronicultura y medio ambiente (pp. 253-276). Sinaloa, México: Colegio de Sonora.
Gómez-Gil, B., Tron-Mayén, L., Roque, A., Turnbull, J. F., Inglis, V., & Guerra-Flores, A. L. (1998). Species of Vibrio spp. isolated from hepatopancreas, hemolymph and digestive tract of a population of health juvenile Penaeus vannamei.Aquaculture, 163(1-2), 1-9.
Hossain, M. S., Aktaruzzaman, M., Fakhruddin, A. N. M., & Uddin, M. J. (2012). Antimicrobial susceptibility of Vibrio species isolated from brackish water shrimp culture environment. Journal of Bangladesh Academic of Science, 36(2), 213-220.
Hossain, M. T., Kim, Y. O., & Kong, I. S. (2013). Multiplex PCR for the detection and differentiation of Vibrio parahaemolyticus strains using the groEL, tdh and trh genes. Molecular and Cellular Probes, 27(5-6), 171-175.
Jayasree, L., Janakiram, P., & Madhavi, R. (2006). Characterization of Vibrio spp. associated with diseased shrimp from culture ponds of Andhra Pradesh (India). Journal of the World Aquaculture Society, 37(4), 523-532.
Kumar, B. K., Deekshit, V. K., Raj, J. R. M., Rai, P., Shivanagowda, B. M., Karunasagar, I., & Karunasagar, I. (2014). Diversity of Vibrio parahaemolyticus associated with disease outbreak among cultured Litopenaeus vannamei (Pacific white shrimp) in India. Aquaculture, 433, 247-251.
Leyton, Y., & Riquelme, C. (2008). Vibrio en los sistemas marinos costeros. Revista de Biología Marina y Oceanografía, 43(3), 441-456.
Li, E., Chen, L., Zeng, C., Chen, X., Yu, N., Lai, Q., & Qin, J. G. (2007). Growth, body composition, respiration and ambient ammonia nitrogen tolerance of juvenile white shrimp, Litopenaeus vannamei, at different salinities. Aquaculture, 265(1-4), 385-390.
Li, E., Chen, L., Zeng, C., Yu, N., Xiong, Z., Chen, X., & Qin, J. G. (2008). Comparison of digestive and antioxidant enzymes activities, haemolymph oxyhemocianin contents and hepatopancreas histology of white shrimp, Litopenaeus vannamei, at various salinities. Aquaculture, 274, 80-86.
Lira, J. R., Amaral, R., Moura, V. F., Rocha, L., & Correia, E. S. (mayo, 2003). Performance evaluation of Litopenaeus vannamei marine shrimp in intensive and semi-intensive farming systems. World Aquaculture Society 2003. Salvador, Bahia, Brazil, May 19-23, 423-424.
Organización de las Naciones Unidas para la Alimentación y la Agricultura. (2014). El estado mundial de la pesca y la acuicultura: Oportunidades y desafíos (pp. 20-25). Roma, Italia: Autor.
Pan, L., Zhang, L., & Liu, H. Y. (2007). Effects of salinity and pH on ion-transport enzyme activities, survival and growth of Litopenaeus vannamei postlarvae. Aquaculture, 273(4), 711-720.
Parvathi, A., George, J., & Kumar, S. (2009). Prevalence and heterogeneity of haemolysin gene vhh among hatchery isolates of Vibrio harveyi in India. Current Microbiology, 59(1), 42-47.
Paydar, M., Shuan, J. T. C., & Lin, T. K. (2013). Prevalence and characterisation of potentially virulent Vibrio parahaemolyticus in seafood in Malaysia using conventional methods, PCR and REP-PCR. Food Control, 32(1), 13-18.
Ray, A. J., Dillon, K. S., & Lotz, J. M. (2011). Water quality dynamics and shrimp (Litopenaeus vannamei) production in intensive, mesohaline culture systems with two levels of biofloc management. Aquacultural Engineering, 45(3), 127-136.
Ray, A. J., Lewis, B. L., Browdy, C. L., & Leffler, J. W. (2010). Suspended solids removal to improve shrimp (Litopenaeus vannamei) production and an evaluation of a plant-based feed in minimal-exchange, superintensive culture systems. Aquaculture, 299(1-4), 89-98.
Sani, N. A., Ariyawansa, S., Babji, A. S., & Hashim, J. K. (2013). The risk assessment of Vibrio parahaemolyticus in cooked black tiger shrimps (Penaeus monodon) in Malaysia. Food Control, 31(2), 546-552.
Shveitzer, R., Arantes, R., Costódio, P. F. S., do Espíritu Santo, C. M., Arana, L. V., Seiffert, W. Q., & Adreatta, E. R. (2013). Effect of different biofloc levels on microbial activity, water quality and performance of Litopenaeus vannamei in a tank system operated with no water exchange. Aquacultural Engineering, 56, 59-70.
Soonthornchai, W., Rungrassamee, W., Karoonuthaisiri, N., Jarayabhand, P., Klinbunga, S., Soderhall, K., & Jiravanichpaisal, P. (2010). Expression of immune-related genes in the digestive organ of shrimp, Penaeus monodon, after an oral infection by Vibrio harveyi. Developmental & Comparative Immunology, 34(1), 19-28.
Wong, S. K., Zhang, X. H., & Woo, N. Y. S. (2012). Vibrio alginolyticus thermolabile hemolysin (TLH) induces apoptosis, membrane vesiculation and necrosis in sea bream erythrocytes. Aquaculture, 330-333, 29-36.
Wu, J. P., Chen, H. C., & Huang, D. J. (2009). Histopathological alterations in gills of white shrimp, Litopenaeus vannamei (Boone) after acute exposure to cadmium and zinc. Bulletin of Environmental Contamination and Toxicology, 82(1), 90-95.
Zhang, X. H., & Austin, B. (2005). Review: Haemolysins in Vibrio species. Journal of Applied Microbiology, 98(5), 1011-1019.
Zhang, X. J., Yan, B. L., Bai, X. S., Bi, K. R., Gao, H., & Qin, G. M. (2014). Isolation and characterization of Vibrio parahaemolyticus and Vibrio rotiferianus associated with mass mortality of Chinese shrimp (Fenneropenaeus chinensis). Journal of Shellfish Research, 33(1), 61-68.
Zhou, Q., Li, K., Jun, X., & Bo, L. (2009). Role and functions of beneficial microorganisms in sustainable aquaculture. Bioresource Technology, 100(16), 3780-3786.
Zorriehzahra, M. J., & Banaederakhshan, R. (2015). Early Mortality Syndrome (EMS) as new emerging threat in shrimp industry. Advances in Animal and Veterinary Sciences, 3(2s), 64-72.
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