Implementation of a system to evaluate neurotoxicity of environmental pollutants in zebrafish larvae (Danio rerio)
DOI:
https://doi.org/10.33064/iycuaa2018741718Keywords:
zebrafish, neurotoxicity, arsenic, atrazine, thiametoxam, pesticidesAbstract
Currently, a wide variety of pollutants are detected in the environment with implications for both health and biodiversity, its high number has made it difficult to study with in vitro and in vivo methods of experimentation. A low cost system was implemented to evaluate the toxic effects of environmental pollutants, using the motor activity as neurotoxicity marker in zebrafish model. Embryos were exposed to inorganic arsenic (Asi) 0.05, 0.5, 5, 50 mg/l, atrazine (ATR) 0.03, 0.3, 3, 10 mg/l and thiamethoxam (TMX) 14.5, 29.1, 43.6 mg/l. Motor activity increased in larvae exposed to ATR and Asi and decreased with the high dose of TMX. These data suggest that system is sensitive for to detect changes in motor behavior in zebrafish larvae, which may contribute to increase the study of environmental pollutants with neurotoxic potential.
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