Detección de vibrio mediante la amplificación de genes de patogenicidad en camarón Litopenaeus vannamei cultivado en un sistema tipo invernadero
DOI:
https://doi.org/10.33064/iycuaa201772218Palabras clave:
Vibrio spp., Vibrio harveyi, gen toxR, gen hemolisina, Litopenaeus vannameiResumen
Las infecciones por vibrio son un problema importante en el cultivo de camarón por la alta mortalidad y grandes pérdidas económicas provocadas. El objetivo del estudio fue identificar genes de patogenicidad
en bacterias vibrio aisladas de camarón L. vannamei cultivado en un sistema intensivo tipo invernadero con nulo recambio de agua. Se determinó cuenta total bacteriana y de vibrio en hemolinfa, hepatopáncreas y branquias de camarón, de donde se obtuvieron aislados bacterianos para la identificación por PCR de Vibrio ssp., V. harveyi gen de hemolisina (vhh), V. harveyi toxR y otras especies patógenas de vibrio. Se identificaron ambos genes (hemolisina y toxR) en cuatro de los aislados de camarón. Esta metodología puede ser una herramienta en la detección de V. harveyi para su prevención y control. No se detectó V. cholerae (V. chol), V. vulnificus (Vvh-785) y V. harveyi (LuxN).
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