In vitro anti-inflammatory activity of the ethanolic and hexanic extracts of Cuscuta jalapensis Schltdl.'s stems
DOI:
https://doi.org/10.33064/iycuaa2018751766Keywords:
anti-inflammatory, membrane stability, erythrocyte, lysosome, extractAbstract
In this work, the in vitro anti-inflammatory activity of ethanolic and hexanic extracts of Cuscuta jalapensis Schltdl. was evaluated by using the erythrocyte membrane stability model against two agents inducing hemolysis, the hypotonic saline solution and heat. A phytochemical sieve was used to determine the families of secondary metabolites present in both extracts being alkaloids and flavonoid principally. A concentration response curve of the antiinflammatory activity was drawn, starting from 50, 100, 200 and 400 μg/ml, of both extracts, using 100μg/ml indomethacin as the pharmacological control. The results indicated that all tested concentrations of the different extracts decreased hemolysis and therefore produced a membrane stabilizing effect. For evaluation with hypotonic saline the ethanolic extract had an effect equal to indimetacin 100 μg/ ml, the concentrations of the hexane extract showed a lower anti-inflammatory activity in this test
Downloads
Metrics
References
• Anilkumar, M., & Johny, J. (2015). Evaluation of in vitro anti-Inflammatory activity of the methanolic extract of Litsea quinqueflora (Dennst.) Suresh. Journal of Pharmacy and Biological Sciences, 10(2), 32-36.
• Barbosa-Filho, J. M., Piuvezam, M. R., Moura, M. D., Silva, M. S., Batista Lima, K. V., Leitão da-Cunha, E. V., …, & Takemura, O. S. (2006). Anti-inflammatory activity of alkaloids: A twentycentury review. Revista Brasileira de Farmacognosia, 16(1), 109-139.
• Buck, H. T. (1969). Convolvulaceae. En A. Burkart (Dir.), Flora Ilustrada de Entre Ríos (Argentina) (Vol. 6, pp. 148-194). Argentina: Colección Científica del Instituto Nacional de Tecnología Agropecuaria.
• Cseke, L. J., Kirakosyan, A., Kaufman, P. B., Warber, S. L., Duke, J. A., & Brielmann, H. L. (2006). Natural Products from Plants (2a. ed.). Boca Raton, FL, US: CRC Press.
• Gaona Fernández, L. A. (2011). Efecto de árnica en un modelo in vitro con citoquinas proinflamatorias y antiinflamatorias. Fase II (Tesis doctoral no publicada). Universidad Nacional de Colombia, Colombia.
• Instituto Nacional de Estadística y Geografía. (2016). Sociodemográfico
de México. México: Autor.
• Kardile, M. V., Mahajan, U. B., Shaikh, H. M., Goyal, S. N., & Patil, C. R. (2016). Membrane stabilization assay for anti-inflammatory activity yields false positive results for samples containing traces of ethanol and methanol. World Journal of Pharmacy and Pharmaceutical Sciences, 5(3), 493-497.
• Kumar, S., Bajwa, B. S., Kuldeep, S., & Kalia, A. N. (2013). Antiinflammatory activity of herbal plants: A review. International Journal of Advances in Pharmacy, Biology and Chemistry, 2(2), 272-281.
• Liao, J. C., Chang, W. T., Lee, M. S., Chiu, Y. J., Chao, W. K., Lin, Y. C., …, Peng, W. H. (2014). Antinociceptive and anti-inflammatory activities of Cuscuta chinensis seeds in mice. The American Journal of Chinese Medicine, 42(1), 223-242.
• Mercy Margaret, T., Krishna, P., Revathi, B., Eswar Tony, D., Sathish Kumar, M., & Narendra Babu, A. (2013). Assessment of in vitro anti inflammatory activity of aqueous extract of Ipomoea batatas tubers. Asian Journal of Research in Biological and Pharmaceutical Sciences, 1(1), 47-53.
• Mohamed Saleem, T. K., Azeem, A. K., Dilip, C., Sankar, C., Prasanth, N. V., & Duraisami, R. (2011). Anti-inflammatory activity of the leaf extacts of Gendarussa vulgaris Nees. Asian Pacific Journal of Tropical Biomedicine, 1(2), 147-149.
• Múlgura, M. E. (1969). Cuscutaceae. En A. Burkart (Dir.), Flora Ilustrada de Entre Ríos (Argentina) (Vol. 6 5a, pp. 195-203). Argentina: Colección Científica del Instituto Nacional de Tecnología Agropecuaria.
• Oxelman, B., Kornhall, P., Olmstead, R. G., & Bremer, B. (2005). Further disintegration of Scrophulariaceae. Taxon, 54, 411-425.
• Rzedowski Rotter, J., & Calderón de Rzedowski, G. (Eds.). (2005). Flora del bajío y de regiones adyacentes (serie de fascículos). Pátzcuaro, Michoacán, México: INECOL.
• Sánchez, P. T., Sirera, R., Peiró, G., & Palmero, F. (2008). Estrés, depresión, inflamación y dolor. R.E.M.E. (Revista Electrónica de Motivación y Emoción), 11(28). Recuperado de http://reme.uji.es/articulos/numero28/article1/article1.pdf
• Shinde, U. A., Phadke, A. S., Nair, A. M., Mungantiwar, A. A., Dikshit, V. J., & Saraf, M. N. (1999). Membrane stabilizing activity-a possible mechanism of action for the anti-inflammatory activity of Cedrus deodara wood oil. Fitoterapia, 70(3), 251-257.
• Sikder, M. A., Rahman, M. A., Kaisar, M. A., Rahman, M. S., Hasan, C. M., & Rashid, M. A. (2011). In vitro antioxidant, reducing power, free radical scavenging and membrane stabilizing activities of seeds of Syzygium cumini L. Latin American Journal of Pharmacy, 30(4), 781-785.
• Souto, A. L., Tavares, J. F., Da Silva, M. S., Diniz, M. D. F., De Athayde-
Filho, P. F., & Barbosa Filho, J. M. (2011). Anti-inflammatory activity of alkaloids: An update from 2000 to 2010. Molecules, 16(10), 8515-8534.
• Susunaga-Notario, A. C., Pérez-Gutiérrez, S., Zavala-Sánchez, M. A., Almanza-Pérez, J. C., Gutiérrez-Carrillo, A., Arrieta-Báez, D., …, Alarcón-Aguilar, F. J. (2014). Bioassay-guided chemical study of the anti-inflammatory effect of Senna villosa (Miller) H. S. Irwin & Barneby (Leguminosae) in TPA-induced ear edema. Molecules, 19(7), 10261-10278.
• Torres Cruz, E., Soto Ojeda, G. A., Palomino Asencio, L., & García Hernández, E. (2017). Estudio ab initio de la capacidad antioxidante de una familia de alcaloides imidazólicos versus flavonoides. Investigación y Ciencia de la Universidad Autónoma de Aguascalientes, 25(72), 30-35.
• Velásquez Gómez, S. V., & Posada Tabares, V. (2013). Actividad anti-inflamatoria in vitro de los extractos y fracciones obtenidas de la corteza interna de Tabebuia chrysantha (JACQ.) G. Nicholson (Tesis doctoral, Universidad Tecnológica de Pereira, Colombia). Recuperada de https://books.google.com.mx/books/about/Actividad_anti_inflamatoria_in_vitro_de.html?id=XF1-swEACAAJ&redir_esc=y
Downloads
Published
License
Las obras publicadas en versión electrónica de la revista están bajo la licencia Creative Commons Atribución-NoComercial-CompartirIgual 4.0 Internacional (CC BY-NC-SA 4.0)
