In vitro fertilization (IVF) of oocytes obtained from sheep using the laparoscopic ovum pick up technique
DOI:
https://doi.org/10.33064/iycuaa2020813137Keywords:
sheep, oocytes, embryos, biotech, maturation, laparoscopyAbstract
In order to fine-tune the laparoscopic ovum pick up (LOPU) technique and develop an in vitro fertilization (IVF) protocol, two groups of sheep were synchronized (vaginal sponges 14 days) and superovulated (FSHp), beginning 60 hours before each LOPU (3 sessions). Laparoscopic follicular aspiration was performed 12 hours after the last dose of FSHp. The overall recovery rate of oocytes in three LOPU sessions in G2 and G1 was 41.0 ± 2.5 and 24.0 ± 2.4% SEM, respectively, presenting differences (P<0.05) in favor of G2. In vitro oocyte culture at 48 hours showed a difference (P<0.05) in favor of G1 (9.7 %) with respect to G2 (0.0).
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Copyright (c) 2020 Julio Ramón Ugalde, Deneb Cervera Paúl, Álvaro Domínguez Rebolledo, Juan Baeza-Rodríguez, Luis Pinzón-López, Roberto Zamora-Bustillos
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