This is an outdated version published on 2021-09-23. Read the most recent version.

In vitro fertilization (IVF) of oocytes obtained from sheep using the laparoscopic ovum pick up technique

Authors

Julio Ramón Ugalde Instituto Tecnológico de Conkal http://orcid.org/0000-0002-9312-8438
Deneb Cervera Paúl Instituto Tecnológico de Conkal http://orcid.org/0000-0002-4508-9462
Álvaro Domínguez Rebolledo Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias campo experimental Mocochá http://orcid.org/0000-0002-1444-3844
Juan Baeza-Rodríguez Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias campo experimental Mocochá http://orcid.org/0000-0001-9339-9695
Luis Pinzón-López Tecnológico Nacional de México campus Conkal http://orcid.org/0000-0002-8405-3948
Roberto Zamora-Bustillos Tecnológico Nacional de México campus Conkal http://orcid.org/0000-0002-4502-1492

DOI:

https://doi.org/10.33064/iycuaa2020813137

Keywords:

sheep, oocytes, embryos, biotech, maturation, laparoscopy

Abstract

In order to fine-tune the laparoscopic ovum pick up (LOPU) technique and develop an in vitro fertilization (IVF) protocol, two groups of sheep were synchronized (vaginal sponges 14 days) and superovulated (FSHp), beginning 60 hours before each LOPU (3 sessions). Laparoscopic follicular aspiration was performed 12 hours after the last dose of FSHp. The overall recovery rate of oocytes in three LOPU sessions in G2 and G1 was 41.0 ± 2.5 and 24.0 ± 2.4% SEM, respectively, presenting differences (P<0.05) in favor of G2. In vitro oocyte culture at 48 hours showed a difference (P<0.05) in favor of G1 (9.7 %) with respect to G2 (0.0).

Downloads

Download data is not yet available.

Metrics

Metrics Loading ...

References

• Alberio, R., Olivera, J., Roche, A., Alabart, J., & Folch, J. (2002). Performance of a modiﬁed ovum pick-up system using three different FSH stimulation protocols in ewes. Small Ruminant Research, 46(2-3), 81-87.

• Baldassarre, H. (2007). Aplicaciones prácticas de la producción de embriones in vitro en la especie caprina. Revista Brasileira de Reprodução Animal, 31(2), 261-267.

• Baldassarre, H., Wang, B., Pierson, J., Neveu, N., Sneek, L., Lapointe, J., … Karatzas, C. N. (2004). Prepubertal propagation of transgenic cloned goats by laparoscopic ovum pick-up and in vitro embryo production. Cloning and Stem Cells, 6(1), 25-29. doi: 10.1089/15362300460743808

• Brackett, B. G., Bousquet, D., Boice, M. L., Donawick, W. J., Evans, J. F., & Dressel, M. A. (1982). Normal development following in vitro fertilization in the cow. Biology of Reproduction, 27(1), 147-158. doi: 10.1095/biolreprod27.1.147

• Chang, M. C. (1959). Fertilization of rabbit ova in vitro. Nature, 184, 466-467.

• Cox, J. F., & Alfaro, V. (2007). In vitro fertilization and development of OPU derived goat and sheep oocytes. Reproduction in Domestic Animals, 42(1), 83-87. doi:10.1111/j.1439-0531.2006.00735.x

• Domínguez Rebolledo, A., Vargas Manzanero, G., Alcaraz Romero, A., Quintal Franco, J., Baeza Rodríguez, J., Rivera Lorca, J., & Ramón Ugalde, J. (2017). Follicular population at the onset of a superovulatory treatment and ovarian response in hair ewes. Romanian Biotechnological Letters, 22(2), 12427-12431.

• Hammami, S. (2008). Producción de embriones ovinos in vitro con vistas a la selección genética (Tesis de maestría). Instituto Agronómico Mediterráneo de Zaragoza, Zaragoza.

• Instituto Nacional para el Federalismo y el Desarrollo Municipal & Gobierno del Estado de Yucatán. (2005). Enciclopedia de los Municipios y Delegaciones de México. Estado de Yucatán [Página electrónica]. Recuperado de http://www.inafed.gob.mx/work/enciclopedia/EMM31yucatan/index.html

• Juárez-Pérez, A., Domínguez-Rebolledo, A., Pinzón-López, L., Aguilar-Urquizo, E., Rivera-Lorca, J., & Ramón-Ugalde, J. (2018). Estacionalidad reproductiva en ovejas tropicales superovuladas. Agroproductividad, 11(10), 133-135.

• Khodaei, M., Shahneh, A. Z., Daliri, M., Kohram, H., & Gharagozlou, F. (2008). In vitro maturation of sheep oocytes in different concentrations of mare serum. African Journal of Biotechnology, 7(18), 3380-3382.

• Koeman, J., Keefer, C. L., Baldassarre, H., & Downey, B. R. (2003). Developmental competence of prepubertal and adult goat oocytes cultured in semi-defined media following laparoscopic recovery. Theriogenology, 60(5), 879-889. doi: 10.1016/s0093-691x(03)00090-6

• Martino, A., Mogas, T., Palomo, M. J., & Paramio, M. T. (1994). Meiotic competence of prepubertal goat oocytes. Theriogenology, 41(4), 969-980.

• O’Brien, J. K., Catt, S. L., Ireland, K. A., Maxwell, W. M., & Evans, G. (1997). In vitro and in vivo developmental capaclty of oocytes from prepubertal and adult sheep. Theriogenology, 47(7), 1433-1443. doi: 10.1016/S0093-691X(97)00134-9

• Palma, G. (2008). Producción in vitro de embriones. En Biotecnología de la reproducción (cap. 16, pp. 3133-3175).

• Pierson, J., Wang, B., Neveu, N., Sneek, L., Côté, F., Karatzas, C. N., & Baldassarre, H. (2004). Effects of repetition, interval between treatments and season on the results from laparoscopic ovum pick-up in goats. Reproduction Fertility and Development, 16(8), 795-799. doi: 10.1071/rd04066

• Ramón-Ugalde, J. P., Folch, J., Cocero, M. J., Piña-Aguilar, R. E., & Alabart, J. L. (2008). Embryo recovery from the oviduct in superovulated ewes: a method to improve MOET systems. Czech Journal of Animal Science, 53(4), 145-151.

• Revel, F., Mermillod, P., Peynot, N., Renard, J. P., & Heyman, Y. (1995). Low developmental capacity of in vitro matured and fertilized oocytes from calves compared with that of cows. Journal of Reproduction and Fertility, 103(1), 115-120. doi:

1530/jrf.0.1030115

• Romaguera, R., Chacaltana, A., Fonseca, C., Anguita, B., Catalá, M., Casanovas, A., …Paramio, M. T. (2007). Utilización de la LOPU en caprino: Valores foliculares y ovocitarios. ITEA, Extra 28(Tomo 1), 33-35.

• Shirazi, A., Shams-Esfandabadi, N. S., & Hosseini, S. M. (2005). A comparison of two recovery methods of ovine oocytes for in vitro maturation. Small Ruminant Research, 58(3), 283-286. doi: 10.1016/j.smallrumres.2004.11.002

• Thibier, M. (1990). New technologies in cattle reproduction. Proceedings of the Regional Seminar held by the International Foundation for Science (IFS), Niamey, Niger. (pp. 247-262).

• Tibary, A., Anouassi, A., & Khatir, H. (2005). Update on reproductive biotechnologies in small ruminants and camelids. Theriogenology 64(3), 618-638. doi: 10.1016/j.theriogenology.2005.05.016

Downloads

Published

2020-11-30 — Updated on 2021-09-23

Versions

How to Cite

Ugalde, J. R., Cervera Paúl, D., Domínguez Rebolledo, Álvaro, Baeza-Rodríguez, J., Pinzón-López, L., & Zamora-Bustillos, R. (2021). In vitro fertilization (IVF) of oocytes obtained from sheep using the laparoscopic ovum pick up technique. Investigación Y Ciencia De La Universidad Autónoma De Aguascalientes, (81), 15–23. https://doi.org/10.33064/iycuaa2020813137 (Original work published November 30, 2020)