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In vitro fertilization (IVF) of oocytes obtained from sheep using the laparoscopic ovum pick up technique
DOI:
https://doi.org/10.33064/iycuaa2020813137Keywords:
sheep, oocytes, embryos, biotech, maturation, laparoscopyAbstract
In order to fine-tune the laparoscopic ovum pick up (LOPU) technique and develop an in vitro fertilization (IVF) protocol, two groups of sheep were synchronized (vaginal sponges 14 days) and superovulated (FSHp), beginning 60 hours before each LOPU (3 sessions). Laparoscopic follicular aspiration was performed 12 hours after the last dose of FSHp. The overall recovery rate of oocytes in three LOPU sessions in G2 and G1 was 41.0 ± 2.5 and 24.0 ± 2.4% SEM, respectively, presenting differences (P<0.05) in favor of G2. In vitro oocyte culture at 48 hours showed a difference (P<0.05) in favor of G1 (9.7 %) with respect to G2 (0.0).
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References
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• Baldassarre, H., Wang, B., Pierson, J., Neveu, N., Sneek, L., Lapointe, J., … Karatzas, C. N. (2004). Prepubertal propagation of transgenic cloned goats by laparoscopic ovum pick-up and in vitro embryo production. Cloning and Stem Cells, 6(1), 25-29. doi: 10.1089/15362300460743808
• Brackett, B. G., Bousquet, D., Boice, M. L., Donawick, W. J., Evans, J. F., & Dressel, M. A. (1982). Normal development following in vitro fertilization in the cow. Biology of Reproduction, 27(1), 147-158. doi: 10.1095/biolreprod27.1.147
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• Cox, J. F., & Alfaro, V. (2007). In vitro fertilization and development of OPU derived goat and sheep oocytes. Reproduction in Domestic Animals, 42(1), 83-87. doi:10.1111/j.1439-0531.2006.00735.x
• Domínguez Rebolledo, A., Vargas Manzanero, G., Alcaraz Romero, A., Quintal Franco, J., Baeza Rodríguez, J., Rivera Lorca, J., & Ramón Ugalde, J. (2017). Follicular population at the onset of a superovulatory treatment and ovarian response in hair ewes. Romanian Biotechnological Letters, 22(2), 12427-12431.
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• Khodaei, M., Shahneh, A. Z., Daliri, M., Kohram, H., & Gharagozlou, F. (2008). In vitro maturation of sheep oocytes in different concentrations of mare serum. African Journal of Biotechnology, 7(18), 3380-3382.
• Koeman, J., Keefer, C. L., Baldassarre, H., & Downey, B. R. (2003). Developmental competence of prepubertal and adult goat oocytes cultured in semi-defined media following laparoscopic recovery. Theriogenology, 60(5), 879-889. doi: 10.1016/s0093-691x(03)00090-6
• Martino, A., Mogas, T., Palomo, M. J., & Paramio, M. T. (1994). Meiotic competence of prepubertal goat oocytes. Theriogenology, 41(4), 969-980.
• O’Brien, J. K., Catt, S. L., Ireland, K. A., Maxwell, W. M., & Evans, G. (1997). In vitro and in vivo developmental capaclty of oocytes from prepubertal and adult sheep. Theriogenology, 47(7), 1433-1443. doi: 10.1016/S0093-691X(97)00134-9
• Palma, G. (2008). Producción in vitro de embriones. En Biotecnología de la reproducción (cap. 16, pp. 3133-3175).
• Pierson, J., Wang, B., Neveu, N., Sneek, L., Côté, F., Karatzas, C. N., & Baldassarre, H. (2004). Effects of repetition, interval between treatments and season on the results from laparoscopic ovum pick-up in goats. Reproduction Fertility and Development, 16(8), 795-799. doi: 10.1071/rd04066
• Ramón-Ugalde, J. P., Folch, J., Cocero, M. J., Piña-Aguilar, R. E., & Alabart, J. L. (2008). Embryo recovery from the oviduct in superovulated ewes: a method to improve MOET systems. Czech Journal of Animal Science, 53(4), 145-151.
• Revel, F., Mermillod, P., Peynot, N., Renard, J. P., & Heyman, Y. (1995). Low developmental capacity of in vitro matured and fertilized oocytes from calves compared with that of cows. Journal of Reproduction and Fertility, 103(1), 115-120. doi:
10.1530/jrf.0.1030115
• Romaguera, R., Chacaltana, A., Fonseca, C., Anguita, B., Catalá, M., Casanovas, A., …Paramio, M. T. (2007). Utilización de la LOPU en caprino: Valores foliculares y ovocitarios. ITEA, Extra 28(Tomo 1), 33-35.
• Shirazi, A., Shams-Esfandabadi, N. S., & Hosseini, S. M. (2005). A comparison of two recovery methods of ovine oocytes for in vitro maturation. Small Ruminant Research, 58(3), 283-286. doi: 10.1016/j.smallrumres.2004.11.002
• Thibier, M. (1990). New technologies in cattle reproduction. Proceedings of the Regional Seminar held by the International Foundation for Science (IFS), Niamey, Niger. (pp. 247-262).
• Tibary, A., Anouassi, A., & Khatir, H. (2005). Update on reproductive biotechnologies in small ruminants and camelids. Theriogenology 64(3), 618-638. doi: 10.1016/j.theriogenology.2005.05.016
• Baldassarre, H. (2007). Aplicaciones prácticas de la producción de embriones in vitro en la especie caprina. Revista Brasileira de Reprodução Animal, 31(2), 261-267.
• Baldassarre, H., Wang, B., Pierson, J., Neveu, N., Sneek, L., Lapointe, J., … Karatzas, C. N. (2004). Prepubertal propagation of transgenic cloned goats by laparoscopic ovum pick-up and in vitro embryo production. Cloning and Stem Cells, 6(1), 25-29. doi: 10.1089/15362300460743808
• Brackett, B. G., Bousquet, D., Boice, M. L., Donawick, W. J., Evans, J. F., & Dressel, M. A. (1982). Normal development following in vitro fertilization in the cow. Biology of Reproduction, 27(1), 147-158. doi: 10.1095/biolreprod27.1.147
• Chang, M. C. (1959). Fertilization of rabbit ova in vitro. Nature, 184, 466-467.
• Cox, J. F., & Alfaro, V. (2007). In vitro fertilization and development of OPU derived goat and sheep oocytes. Reproduction in Domestic Animals, 42(1), 83-87. doi:10.1111/j.1439-0531.2006.00735.x
• Domínguez Rebolledo, A., Vargas Manzanero, G., Alcaraz Romero, A., Quintal Franco, J., Baeza Rodríguez, J., Rivera Lorca, J., & Ramón Ugalde, J. (2017). Follicular population at the onset of a superovulatory treatment and ovarian response in hair ewes. Romanian Biotechnological Letters, 22(2), 12427-12431.
• Hammami, S. (2008). Producción de embriones ovinos in vitro con vistas a la selección genética (Tesis de maestría). Instituto Agronómico Mediterráneo de Zaragoza, Zaragoza.
• Instituto Nacional para el Federalismo y el Desarrollo Municipal & Gobierno del Estado de Yucatán. (2005). Enciclopedia de los Municipios y Delegaciones de México. Estado de Yucatán [Página electrónica]. Recuperado de http://www.inafed.gob.mx/work/enciclopedia/EMM31yucatan/index.html
• Juárez-Pérez, A., Domínguez-Rebolledo, A., Pinzón-López, L., Aguilar-Urquizo, E., Rivera-Lorca, J., & Ramón-Ugalde, J. (2018). Estacionalidad reproductiva en ovejas tropicales superovuladas. Agroproductividad, 11(10), 133-135.
• Khodaei, M., Shahneh, A. Z., Daliri, M., Kohram, H., & Gharagozlou, F. (2008). In vitro maturation of sheep oocytes in different concentrations of mare serum. African Journal of Biotechnology, 7(18), 3380-3382.
• Koeman, J., Keefer, C. L., Baldassarre, H., & Downey, B. R. (2003). Developmental competence of prepubertal and adult goat oocytes cultured in semi-defined media following laparoscopic recovery. Theriogenology, 60(5), 879-889. doi: 10.1016/s0093-691x(03)00090-6
• Martino, A., Mogas, T., Palomo, M. J., & Paramio, M. T. (1994). Meiotic competence of prepubertal goat oocytes. Theriogenology, 41(4), 969-980.
• O’Brien, J. K., Catt, S. L., Ireland, K. A., Maxwell, W. M., & Evans, G. (1997). In vitro and in vivo developmental capaclty of oocytes from prepubertal and adult sheep. Theriogenology, 47(7), 1433-1443. doi: 10.1016/S0093-691X(97)00134-9
• Palma, G. (2008). Producción in vitro de embriones. En Biotecnología de la reproducción (cap. 16, pp. 3133-3175).
• Pierson, J., Wang, B., Neveu, N., Sneek, L., Côté, F., Karatzas, C. N., & Baldassarre, H. (2004). Effects of repetition, interval between treatments and season on the results from laparoscopic ovum pick-up in goats. Reproduction Fertility and Development, 16(8), 795-799. doi: 10.1071/rd04066
• Ramón-Ugalde, J. P., Folch, J., Cocero, M. J., Piña-Aguilar, R. E., & Alabart, J. L. (2008). Embryo recovery from the oviduct in superovulated ewes: a method to improve MOET systems. Czech Journal of Animal Science, 53(4), 145-151.
• Revel, F., Mermillod, P., Peynot, N., Renard, J. P., & Heyman, Y. (1995). Low developmental capacity of in vitro matured and fertilized oocytes from calves compared with that of cows. Journal of Reproduction and Fertility, 103(1), 115-120. doi:
10.1530/jrf.0.1030115
• Romaguera, R., Chacaltana, A., Fonseca, C., Anguita, B., Catalá, M., Casanovas, A., …Paramio, M. T. (2007). Utilización de la LOPU en caprino: Valores foliculares y ovocitarios. ITEA, Extra 28(Tomo 1), 33-35.
• Shirazi, A., Shams-Esfandabadi, N. S., & Hosseini, S. M. (2005). A comparison of two recovery methods of ovine oocytes for in vitro maturation. Small Ruminant Research, 58(3), 283-286. doi: 10.1016/j.smallrumres.2004.11.002
• Thibier, M. (1990). New technologies in cattle reproduction. Proceedings of the Regional Seminar held by the International Foundation for Science (IFS), Niamey, Niger. (pp. 247-262).
• Tibary, A., Anouassi, A., & Khatir, H. (2005). Update on reproductive biotechnologies in small ruminants and camelids. Theriogenology 64(3), 618-638. doi: 10.1016/j.theriogenology.2005.05.016
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Published
2020-11-30
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How to Cite
Ugalde, J. R., Cervera Paúl, D., Domínguez Rebolledo, Álvaro, Baeza-Rodríguez, J., Pinzón-López, L., & Zamora-Bustillos, R. (2020). In vitro fertilization (IVF) of oocytes obtained from sheep using the laparoscopic ovum pick up technique. Investigación Y Ciencia De La Universidad Autónoma De Aguascalientes, (81), 15–23. https://doi.org/10.33064/iycuaa2020813137
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Copyright (c) 2020 Julio Ramón Ugalde, Deneb Cervera Paúl, Álvaro Domínguez Rebolledo, Juan Baeza-Rodríguez, Luis Pinzón-López, Roberto Zamora-Bustillos
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
Las obras publicadas en versión electrónica de la revista están bajo la licencia Creative Commons Atribución-NoComercial-CompartirIgual 4.0 Internacional (CC BY-NC-SA 4.0)
